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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab190175-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$19.90
|
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Ab190175-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$39.90
|
|
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Ab190175-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$99.90
|
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mAb (IM7); Rat anti Mouse CD44 Antibody (PE); Flow Cytometry
| Product Name | CD44 Rat mAb (PE) - Primary antibody, specific to CD44 |
|---|---|
| Synonyms | Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA |
| Host species | Rat |
| Specificity | CD44 |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells |
| Positive Control | Flow Cytometry: C57BL/6 mouse splenocytes |
| Conjugation | PE |
| Excitation(nm) | Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm) |
| Emission(nm) | 575nm |
| Ex/Em(nm) | Ex:565nm;Em:575nm |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).
CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
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