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CD3 Rat mAb - Primary antibody, specific to CD3, Rat IgG2a

    Application:
  • Activation
  • Flow Cytometry
  • Functional Assay
  • Stimulation
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: KT3
  • Conjugation: Unconjugated
In stock
Item Number
Ab094429
Grouped product items
SKU Size
Availability
Price Qty
Ab094429-10μg
10μg
5
$29.90
Ab094429-50μg
50μg
2
$69.90
Ab094429-100μg
100μg
1
$89.90
Ab094429-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$499.90

mAb (KT3); Rat anti Mouse CD3 Antibody; Flow, in vitro T cell negative selection, in vitro T cell stimulation/activation, ICC, IF; Unconjugated

Basic Description

Product Name CD3 Rat mAb - Primary antibody, specific to CD3, Rat IgG2a
Synonyms CD3 | T3e | CD3epsilon | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surfa
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity CD3
Immunogen CBAT6 mouse thymocytes
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Rat anti Mouse CD3 Antibody, Monoclonal (KT3), could be used for Flow, in vitro T cell negative selection, in vitro T cell stimulation/activation, ICC, IF and so on.

Application:
Flow: Use at an assay dependent concentration.

in vitro T cell negative selection: Use at an assay dependent concentration. 

in vitro T cell stimulation/activation: Use at an assay dependent concentration. 

ICC/IF: Use at an assay dependent concentration.

Protein Function
The CD3 complex mediates signal transduction.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 with CD3 Rat mAb (Ab094429) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 (red) with CD3 Rat mAb (Ab094429) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

Use at an assay dependent concentration.

in vitro T cell negative selection

Use at an assay dependent concentration

in vitro T cell stimulation/activation

Use at an assay dependent concentration

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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