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TIM3/CD366 Rat mAb - Primary antibody, specific to TIM 3/CD366, Rat IgG2a

COA COA
    Application:
  • Animal Model
  • Blocking
  • Flow Cytometry
  • Functional Assay
  • Neutralization
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: RMT3-23
  • Conjugation: Unconjugated
In stock
Item Number
Ab131268
Grouped product items
SKU Size
Availability
 Price Qty
Ab131268-10μg
10μg
5
$29.90
Ab131268-50μg
50μg
2
$69.90
Ab131268-100μg
100μg
1
$99.90
Ab131268-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (RMT3-23); Rat anti Mouse TIM 3/CD366 Antibody; Flow, in vivo TIM-3 neutralization, in vitro TIM-3 blocking; Unconjugated

View related series
Click to view target information: HAVCR2
Accession#: Q8VIM0 (2) CD366 (9) Flow Cytometry (1158) Gene ID: 171285 (2) HAVCR2 (11) Tim3 (6)

Basic Description

Product Name TIM3/CD366 Rat mAb - Primary antibody, specific to TIM 3/CD366, Rat IgG2a
Synonyms Tim3 | TIM-3 | Timd3 | CD366 | FLJ14428 | HAVCR2 | HAVcr-2 | hepatitis A virus cellular receptor 2 | kidney injury molecule-3 | KIM-3 | SPTCL | T cell immunoglobulin mucin 3 | T cell immunoglobulin mucin-3 | T-cell immunoglobulin and mucin domain-containi
Specifications & Purity Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity TIM 3/CD366
Immunogen Recombinant protein of mouse TIM-3
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Validated
Product Description

Rat anti Mouse TIM 3/CD366 Antibody, Monoclonal (RMT3-23), could be used for Flow, in vivo TIM-3 neutralization, in vitro TIM-3 blocking and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo TIM-3 neutralization: Use at an assay dependent concentration.
in vitro TIM-3 blocking: Use at an assay dependent concentration.
Protein Function
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

HAVCR2 Tchem Hepatitis A virus cellular receptor 2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

TIM3/CD366/PV-1 Rat mAb (Ab131268) - Flow Cytometry
Flow Cytometry analysis of RAW 264.7 cells labelling TIM3/CD366 (red) with TIM3/CD366 Rat mAb (Ab131268) at 0.1 μg/mL for 1 hour at 4°C. SA-PE (rp156150) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

Use at an assay dependent concentration.
in vivo TIM-3 neutralization

Use at an assay dependent concentration.

in vitro TIM-3 blocking

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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