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SDHB Mouse mAb - Primary antibody, specific to SDHB, IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: CD02/2D6
  • Conjugation: Unconjugated
In stock
Item Number
Ab156576
Grouped product items
SKU Size
Availability
 Price Qty
Ab156576-10μl
10μl
≥10
$66.90
Ab156576-20μl
20μl
7
$99.90
Ab156576-50μl
50μl
≥10
$199.90
Ab156576-100μl
100μl
≥10
$299.90
Ab156576-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD02/2D6); Mouse anti SDHB Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: SDHB
Accession#:P21912 (3) Flow Cytometry (1158) Gene ID:6390 (3) SDHB (3)

Basic Description

Product Name SDHB Mouse mAb - Primary antibody, specific to SDHB, IgG1
Synonyms SDHB Mouse monoclonal antibody | Anti-SDHB antibody | CWS2 antibody | DHSB_HUMAN antibody | FLJ92337 antibody | Ip antibody | Iron sulfur subunit antibody | Iron sulfur subunit of complex II antibody | Iron-sulfur subunit of complex II antibody | mitochon
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species Mouse
Specificity SDHB
Immunogen Recombinant protein corresponding to Human SDHB
Positive Control WB: HEK293, HepG2, K562, Jurkat, HeLa, HUVEC, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysates Flow: Jurkat cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application

WB: 1/1,000 - 1/20,000 ; Predicted molecular weight: 31 kDa

Flow: 0.16 μg/mL - 5.0 μg/mL

ICC/IF: 2.0 μg/mL

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SDHB Tchem Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

SDHB Mouse mAb (Ab156576) - Western Blot
All lanes: SDHB Antibody (Ab156576) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 31.6 kDa
Observed band size: 29.1 kDa
Exposure time: 80.6 s 

SDHB Mouse mAb (Ab156576) - Western Blot
All lanes: SDHB Antibody (Ab156576) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 31.6 kDa
Observed band size: 28.9 kDa
Exposure time: 80.6 s 

SDHB Mouse mAb (Ab156576) - ICC/IF
IF analysis of SDHB (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with SDHB Mouse mAb (Ab156576) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

SDHB Mouse mAb (Ab156576) - Flow Cytometry
Overlay histogram showing HeLa cells stained with SDHB Mouse mAb (Ab156576) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue)

Application

Application Dilution info
IF/ICC

5.0 μg/mL
WB

1/1,000 - 1/20,000 ; Predicted molecular weight: 31 kDa
Flow Cytometry

0.16 μg/mL - 5.0 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

6 results found

Lot Number Certificate Type Date Item
ZJ24F0202906 Certificate of Analysis Mar 01, 2024 Ab156576
ZJ24F0202905 Certificate of Analysis Mar 01, 2024 Ab156576
ZJ24F0202904 Certificate of Analysis Mar 01, 2024 Ab156576
ZJ23F1202031 Certificate of Analysis Dec 12, 2023 Ab156576
ZJ23F1202030 Certificate of Analysis Dec 12, 2023 Ab156576
ZJ23F1202029 Certificate of Analysis Dec 12, 2023 Ab156576

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    Recombinant SDHB Antibody

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