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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab191887-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab191887-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$189.90
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Ab191887-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$309.90
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Ab191887-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
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mAb (AB01/2E11); Mouse anti Human Profilin 1; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated
| Product Name | Profilin 1 Mouse mAb - Primary antibody, specific to Profilin 1, Mouse IgG2b |
|---|---|
| Synonyms | Actin binding protein antibody | ALS18 antibody | Epididymis tissue protein Li 184a antibody | OTTHUMP00000125244 antibody | PFN 1 antibody | Pfn antibody | PFN1 antibody | PROF1_HUMAN antibody | Profilin I antibody | Profilin-1 antibody | ProfilinI antib |
| Specifications & Purity | ExactAb™, Validated, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg |
| Host species | Mouse |
| Specificity | Profilin 1 |
| Immunogen | Recombinant protein corresponding to Human Profilin 1 |
| Positive Control | WB: HeLa, HepG2, HT-29, K562, HEK293 and Jurkat whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Profilin 1 Antibody, clone AB01/2E11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | lambda |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s
Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s
Profilin 1 Mouse mAb (Ab191887) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab191887) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).
Profilin 1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Profilin 1 Mouse mAb (Ab191887) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| WB | 1/1,000 - 1/100,000; Predicted molecular weight: 15.0 KDa |
| Flow Cytometry | 1/200 - 1/25000 |
Starting at $69.90
Starting at $66.90