Product Name	I-A/I-E Rat mAb (AF647) - Primary antibody, specific to I-A/I-E, Rat IgG2b
Synonyms	IAb \| Ia2 \| Ia-2 \| Abeta \| H-2Ab \| H2-Ab \| Rmcs1 \| I-Abeta \| Eb \| Ia4 \| H2Eb \| Ia-4 \| H-2Eb \| E-alpha-f \| H-2 class II histocompatibility antigen, E-K alpha chain \| H-2 class II histocompatibility antigen, E-U alpha chain \| H-2E \| H2-Ea \| H-2Ea \| H2-Ea-ps
Specifications & Purity	ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides
Host species	Rat
Specificity	I-A/I-E
Immunogen	Activated C57BL/6 mouse spleen cells
Positive Control	Flow: C57BL/6 mouse splenocytes.
Conjugation	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm
Grade	Em:668nm, Ex:650nm, ExactAb™, Validated

Product Properties

Isotype	Rat IgG2b
Light Chain Type	kappa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: I-A/I-E Rat mAb (AF647) (Ab190267)

I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).

Flow Cytometry: I-A/I-E Rat mAb (AF647) (Ab190267)

I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤0.1 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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I-A/I-E Rat mAb (AF647) - Primary antibody, specific to I-A/I-E, Rat IgG2b

Basic Description

Product Properties

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

I-A/I-E Rat mAb (AF647) - Primary antibody, specific to I-A/I-E, Rat IgG2b

Basic Description

Product Properties

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews