Grouped product items

SKU

Size

Availability

Price

Qty

Ab108247-10μg

10μg

$16.90

Ab108247-50μg

50μg

$49.90

Ab108247-100μg

100μg

$69.90

Ab108247-1mg

1mg

Available within 8-12 weeks(?)

$619.90

Basic Description

Product Name	HLA-DR Mouse mAb - Primary antibody, specific to HLA-DR, Mouse IgG2a
Synonyms	FLJ51114 \| histocompatibility antigen HLA-DR alpha \| HLA class II histocompatibility antigen, DR alpha chain \| HLADR \| HLA-DR \| HLA-DRA \| HLA-DRA1 \| major histocompatibility complex, class II, DR alpha \| MHC cell surface glycoprotein \| MHC class II antige
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species	Mouse
Specificity	HLA-DR
Immunogen	Human lymphoblastoid B-cell line RPMI-8866
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human HLA-DR Antibody, Monoclonal (L243), could be used for Flow and so on. Application: Flow: 0.06 μg/100w cells Protein Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Product Properties

Isotype	Mouse IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)

Discover Target: HLA-DRA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA-DR with HLA-DR Mouse mAb (Ab108247) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA-DR (red) with HLA-DR Mouse mAb (Ab108247) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling HLA-DR (red) with HLA-DR Mouse mAb (Ab108247) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	0.06 μg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0405013	Certificate of Analysis	Apr 29, 2024	Ab108247
ZJ24F0405012	Certificate of Analysis	Apr 29, 2024	Ab108247
ZJ24F0405011	Certificate of Analysis	Apr 29, 2024	Ab108247

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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HLA-DR Mouse mAb - Primary antibody, specific to HLA-DR, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

HLA-DR Mouse mAb - Primary antibody, specific to HLA-DR, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews