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E Cadherin/CD324 Rat mAb - Primary antibody, specific to E Cadherin/CD324, Rat IgG1

    Application:
  • Animal Model
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • IP
  • Neutralization
  • WB
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG1
  • Clone Number: DECMA-1
  • Conjugation: Unconjugated
In stock
Item Number
Ab100726
Grouped product items
SKU Size
Availability
Price Qty
Ab100726-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab100726-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$169.90
Ab100726-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90
Ab100726-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$899.90

mAb (DECMA-1); Rat anti Mouse E Cadherin/CD324 Antibody; in vivo E-Cadherin neutralization, in vitro E-Cadherin neutralization, ICC, IF, IP, WB, Flow; Unconjugated

Basic Description

Product Name E Cadherin/CD324 Rat mAb - Primary antibody, specific to E Cadherin/CD324, Rat IgG1
Synonyms Um | UVO | Ecad | ARC-1 | E-cad | Arc-1 | CAD1 | cadherin 1, E-cadherin (epithelial) | cadherin 1, type 1, E-cadherin (epithelial) | Cadherin-1 | calcium-dependent adhesion protein, epithelial | CAM 120/80 | CD324 antigen | CD324 | CDH1 | CDHE | cell-CAM
Specifications & Purity Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity E Cadherin/CD324
Immunogen Mouse PCC4azal teratocarcinoma cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Validated
Product Description

Rat anti Mouse E Cadherin/CD324 Antibody, Monoclonal (DECMA-1), could be used for in vivo E-Cadherin neutralization, in vitro E-Cadherin neutralization, ICC, IF, IP, WB, Flow and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo E-Cadherin neutralization: Use at an assay dependent concentration. 
in vitro E-Cadherin neutralization: Use at an assay dependent concentration. 
ICC/IF: Use at an assay dependent concentration. 
IP: Use at an assay dependent concentration. 
WB: Use at an assay dependent concentration. 
Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Product Properties

Isotype Rat IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

CDH1 Tbio Cadherin-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

E Cadherin/CD324 Rat mAb (Ab122404) - Flow Cytometry
Flow Cytometry analysis of F9 cells labelling E Cadherin/CD324 (red) with E Cadherin/CD324 Rat mAb (Ab122404) at 0.1 μg/mL for 1 hour at 4°C. Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AF488 at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
WB

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo E-Cadherin neutralization

Use at an assay dependent concentration.

in vitro E-Cadherin neutralization

Use at an assay dependent concentration.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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Solution Calculators

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