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CD47 Rat mAb - Primary antibody, specific to CD47, Rat IgG2a

COA COA
    Application:
  • Animal Model
  • Blocking
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: MIAP301
  • Conjugation: Unconjugated
In stock
Item Number
Ab094972
Grouped product items
SKU Size
Availability
 Price Qty
Ab094972-10μg
10μg
10
$29.90
Ab094972-50μg
50μg
4
$69.90
Ab094972-100μg
100μg
2
$99.90
Ab094972-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (MIAP301); Rat anti Mouse CD47 Antibody; Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC; Unconjugated

View related series
Click to view target information: CD47
Accession#: Q61735 (5) CD47 (19) Flow Cytometry (1158) Gene ID: 16423 (5) IAP (40)

Basic Description

Product Name CD47 Rat mAb - Primary antibody, specific to CD47, Rat IgG2a
Synonyms Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 |
Specifications & Purity Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity CD47
Immunogen Intact CD47 purified from placenta
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Validated
Product Description

Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo CD47 blockade: Use at an assay dependent concentration.
in vitro CD47 blockade: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
Protein Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD47 Rat mAb (Ab094972) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD47 with CD47 Rat mAb (Ab094972) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD47 Rat mAb (Ab094972) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD47 (red) with CD47 Rat mAb (Ab094972) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

Use at an assay dependent concentration.
Flow Cytometry

Use at an assay dependent concentration.
in vivo CD47 blockade

Use at an assay dependent concentration.
in vitro CD47 blockade

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0707950 Certificate of Analysis Jul 08, 2024 Ab094972
ZJ24F0707949 Certificate of Analysis Jul 08, 2024 Ab094972
ZJ24F0707948 Certificate of Analysis Jul 08, 2024 Ab094972

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