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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab185370-10μg
|
10μg |
2
|
$59.90
|
|
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Ab185370-25μg
|
25μg |
2
|
$119.90
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|
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Ab185370-100μg
|
100μg |
1
|
$279.90
|
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mAb (E11); Mouse anti Human CD35 Antibody (Biotin); Flow
| Product Name | CD35 Mouse mAb (Biotin) - Primary antibody, specific to CD35, Mouse IgG1 |
|---|---|
| Synonyms | CD35 Monoclonal Antibody (E11), Biotin | Biotin Anti-Human CD35 Antibody[E11] | CD35 | C3BR | complement component (3b/4b) receptor 1 (Knops blood group) | CR1 | KN | Biotin Mouse Anti-Human CD35 | Biotin anti-human CD35 Antibody | Knops blood group antig |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | CD35 |
| Immunogen | Human CD35 from Acute monocytic leukemia cells and normal blood monocytes. |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Application: |
| Isotype | Mouse IgG1 |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD35 Mouse mAb (Biotin) (Ab185370) - Flow Cytometry
Flow cytometry analysis of CD35 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD35 Mouse mAb (Biotin) (Ab185370) at 0.0015μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).
CD35 Mouse mAb (Biotin) (Ab185370) - Flow Cytometry
Flow cytometry analysis of CD35 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD35 Mouse mAb (Biotin) (Ab185370) at 0.0015μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.05 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.05 μg per million cells in 100 µL staining volume. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 02, 2024 | Ab185370 | |
| Certificate of Analysis | Aug 02, 2024 | Ab185370 | |
| Certificate of Analysis | Aug 02, 2024 | Ab185370 | |
| Certificate of Analysis | Jul 26, 2024 | Ab185370 |
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