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Caspase 3 Mouse mAb - Primary antibody, specific to Human CASP3

COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2a
  • Clone Number: CD01/2F2
  • Conjugation: Unconjugated
In stock
Item Number
Ab156544
Grouped product items
SKU Size
Availability
 Price Qty
Ab156544-10μl
10μl
9
$63.90
Ab156544-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$192.90
Ab156544-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$307.90
Ab156544-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD01/2F2); Mouse anti Human CASP3; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

View related series
Click to view target information: CASP3
Accession#:P42574 (4) CASP3 (4) CPP32 (2) Flow Cytometry (1158) Gene ID:836 (4)

Basic Description

Product Name Caspase 3 Mouse mAb - Primary antibody, specific to Human CASP3
Synonyms A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3 | Caspase-3 subunit p12 antibody | Caspase3 antibody | CC3 antibody | CPP 32 antibody
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many protein
Host species Mouse
Specificity Human CASP3
Immunogen Recombinant protein corresponding to Human CASP3
Positive Control WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver tissue lysates Flow Cytometry: Jurkat cells IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human CASP3 Antibody, clone CD01/2F2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

CASP3 Tchem Caspase-3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34,36 kDa
Exposure time: 16.2 s 

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s 

Caspase 3 Mouse mAb (Ab156544) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with Caspase 3  Mouse mAb (Ab156544) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue). ​

Caspase 3 Mouse mAb (Ab156544) - ICC/IF
IF analysis of Caspase 3 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase 3 Mouse mAb (Ab156544) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1000 - 1/200000
Flow Cytometry

1/200 - 1/12500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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