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Calcein-AM - ≥90% (HPLC), high purity , CAS No.148504-34-1

101 Citations COA COA
    Grade & Purity:
  • ≥90%(HPLC)
In stock
Item Number
C273362
Grouped product items
SKU Size
Availability
 Price Qty
C273362-1mg
1mg
2
$383.90
C273362-5mg
5mg
2
$1,333.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet


View related series
Fluorescent Dye (372)

Basic Description

Synonyms AC1L916S | acetoxymethyl 2-[[2-(acetoxymethoxy)-2-oxo-ethyl]-[[3',6'-diacetoxy-7'-[[bis[2-(acetoxymethoxy)-2-oxo-ethyl]amino]methyl]-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate | Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diace
Specifications & Purity ≥90%(HPLC)
Storage Temp Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description

Calcein, AM is a cell staining reagent that can fluorescently label living cells. After it penetrates the cell membrane and enters the cell, it is cleaved by the intracellular esterase to form Calcein, which is retained in the cell and emits strong green fluorescence. Compared with other similar reagents (such as BCECF, AM and CFDA), Calcein, AM has very low cytotoxicity. The excitation and emission wavelengths of Calcein are 490 nm and 515 nm, respectively.

Calcein, AM only stain live cells. PI, which is a nuclear staining dye, cannot pass through the cell membrane of living cells. It passes through the disordered area of the dead cell membrane to reach the nucleus, and is embedded in the cell’s DNA double helix to generate red fluorescence (excitation: 535 nm, emission: 617 nm). PI only stains dead cells. Since both Calcein and PI-DNA can be excited at 490 nm, a fluorescence microscope can be used to observe live and dead cells simultaneously. With 545 nm excitation, only dead cells can be observed. Based on the above characteristics, Calcein, AM and PI are often combined to double stain live and dead cells.

Due to the different optimal staining conditions for different cell lines, we recommend determining the appropriate concentrations of Calcein, AM and PI individually.

 

Instructions                                                                              

(1) Prepare 1 mM Calcein, AM solution with DMSO, and dilute it with PBS to make 1-50 μM Calcein, AM solution.

(2) Add 1/10 of the volume of the cell culture medium Calcein, AM solution to the cell culture medium. b)

(3) Incubate the cells at 37°C for 15-30 minutes.

(4) Wash the cells twice with PBS or an appropriate buffer.

(5) Observe the cells with a fluorescence microscope with a filter with excitation wavelength of 490 nm and emission wavelength of 515 nm.

a) If it is difficult for Calcein and AM to enter cells, you can use surfactants such as Pluronic F127.

b) It is also possible to use 1/10 concentration of Calcein, AM solution instead of medium.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organoheterocyclic compounds
Class Benzopyrans
Subclass 1-benzopyrans
Intermediate Tree Nodes Dibenzopyrans
Direct Parent Xanthenes
Alternative Parents Alpha amino acid esters  Diarylethers  Phthalides  Benzofuranones  Isobenzofurans  Acylals  Aralkylamines  Benzenoids  Trialkylamines  Lactones  Oxacyclic compounds  Acetals  Carbonyl compounds  Hydrocarbon derivatives  Organic oxides  Organopnictogen compounds  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Xanthene - Alpha-amino acid ester - Diaryl ether - Benzofuranone - Isobenzofuranone - Alpha-amino acid or derivatives - Phthalide - Isocoumaran - Isobenzofuran - Acylal - Aralkylamine - Benzenoid - Carboxylic acid ester - Lactone - Tertiary aliphatic amine - Tertiary amine - Amino acid or derivatives - Acetal - Carboxylic acid derivative - Oxacycle - Ether - Hydrocarbon derivative - Organic nitrogen compound - Carbonyl group - Organic oxide - Organopnictogen compound - Organic oxygen compound - Amine - Organooxygen compound - Organonitrogen compound - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors organooxygen compound

Associated Targets(Human)

A498 (42825 Activities)
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ACHN (49357 Activities)
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CAKI-1 (44928 Activities)
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CCRF-CEM (65223 Activities)
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COLO 205 (50209 Activities)
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DU-145 (51482 Activities)
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HL-60 (67320 Activities)
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HT-29 (80576 Activities)
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K562 (73714 Activities)
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M14 (47487 Activities)
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MCF7 (126967 Activities)
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MOLT-4 (49676 Activities)
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OVCAR-3 (48710 Activities)
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OVCAR-4 (44535 Activities)
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OVCAR-5 (45555 Activities)
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OVCAR-8 (47708 Activities)
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PC-3 (62116 Activities)
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RXF 393 (41971 Activities)
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SF-295 (48000 Activities)
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SK-MEL-2 (46422 Activities)
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SK-MEL-28 (48833 Activities)
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SK-MEL-5 (47095 Activities)
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SK-OV-3 (52876 Activities)
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SN12C (47755 Activities)
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SNB-19 (46794 Activities)
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TK-10 (45540 Activities)
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U-251 (51189 Activities)
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UACC-257 (46019 Activities)
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UACC-62 (47335 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
UO-31 (46270 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
786-0 (47912 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
A549 (127892 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
NCI/ADR-RES (33767 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
T47D (39041 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EKVX (44102 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
NCI-H322M (45589 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HCT-116 (91556 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HOP-92 (41141 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Hs-578T (29457 Activities)
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NCI-H460 (60772 Activities)
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SF-268 (49410 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
IGROV-1 (47897 Activities)
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LOX IMVI (44321 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HOP-62 (47048 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MDA-MB-435 (38290 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MDA-N (28205 Activities)
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Malme-3M (44254 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MDA-MB-231 (73002 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
SNB-75 (44215 Activities)
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NCI-H226 (44470 Activities)
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Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Names and Identifiers

Pubchem Sid 504758620
Pubchem Sid Url https://pubchem.ncbi.nlm.nih.gov/substance/504758620
IUPAC Name acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[[3',6'-diacetyloxy-7'-[[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate
INCHI InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
InChIKey BQRGNLJZBFXNCZ-UHFFFAOYSA-N
Smiles CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Isomeric SMILES CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Molecular Weight 994.87
Reaxy-Rn 10757520
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=10757520&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
I2308218 Certificate of Analysis Sep 11, 2023 C273362
F2306048 Certificate of Analysis Jun 08, 2023 C273362

Chemical and Physical Properties

Sensitivity light sensitive;Moisture sensitive
Molecular Weight 994.900 g/mol
XLogP3 2.900
Hydrogen Bond Donor Count 0
Hydrogen Bond Acceptor Count 25
Rotatable Bond Count 32
Exact Mass 994.249 Da
Monoisotopic Mass 994.249 Da
Topological Polar Surface Area 305.000 Ų
Heavy Atom Count 71
Formal Charge 0
Complexity 1810.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 1

Alternative Products

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Solution Calculators

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