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Aurora A Mouse mAb - Primary antibody, specific to Human AURKA

    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: EF01/3C12
  • Conjugation: Unconjugated
In stock
Item Number
Ab184689
Grouped product items
SKU Size
Availability
Price Qty
Ab184689-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab184689-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab184689-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab184689-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (EF01/3C12); Mouse anti Human AURKA; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name Aurora A Mouse mAb - Primary antibody, specific to Human AURKA
Synonyms AIK antibody | ARK-1 antibody | ARK1 antibody | AURA antibody | Aurka antibody | Aurora 2 antibody | Aurora A antibody | Aurora kinase A antibody | Aurora-related kinase 1 antibody | Aurora/IPL1 like kinase antibody | AURORA/IPL1-like kinase antibody | Au
Specifications & Purity ExactAb™, Carrier Free, Validated, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mit
Host species Mouse
Specificity Human AURKA
Immunogen Recombinant protein corresponding to Human AURKA
Positive Control WB: MCF-7 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human AURKA Antibody, clone EF01/3C12, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

AURKA Tchem Aurora kinase A (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Aurora A Mouse mAb (Ab184689) - Western Blot
All lanes: Aurora A Mouse Antibody (Ab184689) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 42 kDa
Exposure time: 37.3 s 

Aurora A Mouse mAb (Ab184689) - Flow Cytometry ​
Flow Cytometry analysis of HeLa cells stained with Aurora A  Mouse mAb (Ab184689) (Red) at 0.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue). ​

Aurora A Mouse mAb (Ab184689) - IF/ICC
IF analysis of Aurora A (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Aurora A Mouse mAb (Ab184689) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
WB

1/1000 - 1/2000

IF/ICC

1/200

Flow Cytometry

1/1000 - 1/10000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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