Skip to the beginning of the images gallery

Alpha Galactosidase - Specific Activity >30 U/mg；Activity 80 U/ml, high purity

COA

Grade & Purity:

Specific Activity ＞30 U/mg;Activity 80 U/ml

Bioactivity: 400 U/ml

In stock

Item Number

A489917

Grouped product items
SKU	Size	Availability	Price	Qty
A489917-20μl	20μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$739.90
A489917-60μl	60μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,738.90

View related series

Click to view protein information: GLA

Basic Description

Product Name	Alpha Galactosidase - Specific Activity >30 U/mg；Activity 80 U/ml, high purity
Synonyms	α-D-galactoside galactohydrolase, melibiase
Product Description	Product Description Alpha Galactosidase from E. coli cleaves α(1-3)- and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. There is no activity on α(1-4) linked galactose. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with living cells. Molecular Weight ~80,000 daltons Contents Alpha galactosidase in 50 mM sodium phosphate, pH 7.5 included with 20 µL and 60 µl pack sizes: Reaction buffer - 250mM Sodium phosphate, pH 6.5 Specificity Non-reducing terminal alpha-(1-3)- and alpha-(1-6)- galactose. There is no activity on alpha-(1-4)-galactose. Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. Specific Activity One unit of alpha-(1-3,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 25°C pH 6.5 from p-nitrophenyl-alpha-D-galactopyranoside. Purity α(1-3,6) galactosidase is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37°C with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.of the BSA band after SDS-PAGE should show no evidence of degradation. Directions for use 1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube. 2. Add water to 13 µl and 4 µl 5X Reaction Buffer. 3. Add 2 µl alpha-(1-3,6)-Galactosidase. 4. Incubate at 37°C for 1 hour. Longer incubations are necessary if fucose is present on the penultimate sugar. Applications Structural analysis of oligosaccharides Xenograft transplantation studies Removing heterogeneity from glycoproteins
Specifications & Purity	Specific Activity ＞30 U/mg;Activity 80 U/ml
Bioactivity	400 U/ml
Enzyme Commission Number	3.2.1.22

Storage and Shipping

Concentration	Specific Activity ＞30 U/mg;Activity 80 U/ml
Storage Temp	Store at 2-8°C
Shipped In	Wet ice

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration