Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab088099-10μl
|
10μl |
1
|
$69.90
|
|
|
Ab088099-50μl
|
50μl |
1
|
$209.90
|
|
|
Ab088099-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$359.90
|
|
|
Ab088099-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
|
mAb (E7S8); Mouse anti Human ALDH6A1 Antibody; WB, IHC; Unconjugated
| Product Name | ALDH6A1 Mouse mAb - Primary antibody, specific to ALDH6A1, Mouse IgG1 |
|---|---|
| Synonyms | AI314632 antibody | Aldehyde dehydrogenase 6 family member A1 antibody | Aldehyde dehydrogenase family 6 member A1 antibody | Aldehyde dehydrogenase family 6, subfamily A1 antibody | ALDH6A1 antibody | Malonate semialdehyde dehydrogenase antibody | Malona |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA. |
| Host species | Mouse |
| Specificity | ALDH6A1 |
| Immunogen | Recombinant fusion protein of human ALDH6A1. |
| Positive Control | WB: Mouse liver, LNCaP lysates. IHC: Human colon tissue. IF/ICC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human ALDH6A1 Antibody, Monoclonal (E7S8), could be used for WB, IHC, ICC/IF and so on.
|
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 28.1 s
ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 96.3 s
ALDH6A1 Mouse mAb (Ab088099) - IF/ICC
Immunofluorescent analysis of ALDH6A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALDH6A1 Mouse mAb (Ab088099) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ALDH6A1 Mouse mAb (Ab088099) - IHC
Immunohistochemical analysis of ALDH6A1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALDH6A1 Mouse mAb (Ab088099) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| ICC/IF |
1/10-1/50
|
| IHC | 1/50-1/200 |
| WB |
1/500-1/1000
|