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ALDH6A1 Mouse mAb - Primary antibody, specific to ALDH6A1, Mouse IgG1

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG1
  • Clone Number: E7S8
  • Conjugation: Unconjugated
In stock
Item Number
Ab088099
Grouped product items
SKU Size
Availability
Price Qty
Ab088099-10μl
10μl
1
$69.90
Ab088099-50μl
50μl
1
$209.90
Ab088099-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$359.90
Ab088099-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (E7S8); Mouse anti Human ALDH6A1 Antibody; WB, IHC; Unconjugated

Basic Description

Product Name ALDH6A1 Mouse mAb - Primary antibody, specific to ALDH6A1, Mouse IgG1
Synonyms AI314632 antibody | Aldehyde dehydrogenase 6 family member A1 antibody | Aldehyde dehydrogenase family 6 member A1 antibody | Aldehyde dehydrogenase family 6, subfamily A1 antibody | ALDH6A1 antibody | Malonate semialdehyde dehydrogenase antibody | Malona
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Biochemical and Physiological Mechanisms Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Host species Mouse
Specificity ALDH6A1
Immunogen Recombinant fusion protein of human ALDH6A1.
Positive Control WB: Mouse liver, LNCaP lysates. IHC: Human colon tissue. IF/ICC: MCF7 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human ALDH6A1 Antibody, Monoclonal (E7S8), could be used for WB, IHC, ICC/IF and so on.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 28.1 s

ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 96.3 s

ALDH6A1 Mouse mAb (Ab088099) - IF/ICC
Immunofluorescent analysis of ALDH6A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALDH6A1 Mouse mAb (Ab088099) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).


ALDH6A1 Mouse mAb (Ab088099) - IHC
Immunohistochemical analysis of ALDH6A1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALDH6A1 Mouse mAb (Ab088099) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

Application Dilution info
ICC/IF

1/10-1/50

IHC

1/50-1/200

WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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