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Acridine Orange hydrochloride - >99%, high purity , CAS No.65-61-2

    Grade & Purity:
  • ≥99%
In stock
Item Number
A304298
Grouped product items
SKU Size
Availability
Price Qty
A304298-25mg
25mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$121.90
A304298-100mg
100mg
4
$340.90

Nucleic acid binding dye

Basic Description

Synonyms N3,N3,N6,N6-tetramethylacridine-3,6-diamine hydrochloride | acridine orange hydrochloride | Acridine Orange (Hydrochloride)
Specifications & Purity ≥99%
Biochemical and Physiological Mechanisms Cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.
Storage Temp Protected from light,Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:
• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP). 
• or autophagy detection. Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organoheterocyclic compounds
Class Quinolines and derivatives
Subclass Benzoquinolines
Intermediate Tree Nodes Not available
Direct Parent Acridines
Alternative Parents Aminoquinolines and derivatives  Dialkylarylamines  Pyridines and derivatives  Benzenoids  Heteroaromatic compounds  Azacyclic compounds  Organopnictogen compounds  Hydrochlorides  Hydrocarbon derivatives  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Acridine - Aminoquinoline - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Pyridine - Benzenoid - Heteroaromatic compound - Tertiary amine - Azacycle - Organic nitrogen compound - Hydrocarbon derivative - Organonitrogen compound - Organopnictogen compound - Amine - Hydrochloride - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as acridines. These are organic compounds containing the acridine moiety, a linear tricyclic heterocycle which consists of two benzene rings joined by a pyridine ring.
External Descriptors hydrochloride - aminoacridines

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Names and Identifiers

IUPAC Name 3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;hydrochloride
INCHI InChI=1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChIKey VSTHNGLPHBTRMB-UHFFFAOYSA-N
Smiles CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl
Isomeric SMILES CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl
Molecular Weight 301.81
Reaxy-Rn 3575587
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3575587&ln=

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot Number Certificate Type Date Item
K2429523 Certificate of Analysis Nov 20, 2024 A304298
K2429524 Certificate of Analysis Nov 20, 2024 A304298
K2429546 Certificate of Analysis Nov 20, 2024 A304298
G2415813 Certificate of Analysis Jul 05, 2024 A304298
G2416637 Certificate of Analysis Jul 05, 2024 A304298
G2205051 Certificate of Analysis Jun 14, 2022 A304298
G2205052 Certificate of Analysis Jun 14, 2022 A304298
A2425086 Certificate of Analysis Jun 14, 2022 A304298

Chemical and Physical Properties

Solubility Solvent:water, Max Conc. mg/mL: 30.18, Max Conc. mM: 100; Solvent:DMSO, Max Conc. mg/mL: 30.18, Max Conc. mM: 100
Sensitivity Hygroscopic;light sensitive;heat sensitive
Molecular Weight 301.800 g/mol
XLogP3
Hydrogen Bond Donor Count 1
Hydrogen Bond Acceptor Count 3
Rotatable Bond Count 2
Exact Mass 301.135 Da
Monoisotopic Mass 301.135 Da
Topological Polar Surface Area 19.400 Ų
Heavy Atom Count 21
Formal Charge 0
Complexity 298.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 2

Solution Calculators

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