Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab086955-10μl
|
10μl |
1
|
$49.90
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Ab086955-50μl
|
50μl |
1
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$149.90
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Ab086955-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$249.90
|
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Ab086955-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (CHS11A2); Mouse anti Human ABCB5 Antibody; WB, IHC; Unconjugated
| Product Name | ABCB5 Mouse mAb - Primary antibody, specific to ABCB5, Mouse IgG1 |
|---|---|
| Synonyms | ABCB 5 antibody | ABCB5 antibody | ABCB5 P-gp antibody | ABCB5_HUMAN antibody | ABCB5alpha antibody | ABCB5beta antibody | ATP binding cassette protein antibody | ATP binding cassette sub family B (MDR/TAP) member 5 antibody | ATP binding cassette sub fam |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair. |
| Host species | Mouse |
| Specificity | ABCB5 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human ABCB5. |
| Positive Control | WB: HeLa, HEK293 and Jurkat cell lysates. IHC: Human breast cancer tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human ABCB5 Antibody, Monoclonal (CHS11A2), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
ABCB5 Mouse mAb (Ab086955) - Western Blot
All lanes: ABCB5 Mouse mAb (Ab086955) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 86 kDa
Exposure time: 100.0 s
ABCB5 Mouse mAb (Ab086955) - IHC
Immunohistochemical analysis of ABCB5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ABCB5 Mouse mAb (Ab086955) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IHC |
1/200-1/500
|