Basic Description

Synonyms	F04-0012 \| BP-23367 \| F5ITC \| FITC ISOMER I \| FluoresceinisothiocyanateisomerI \| BP-30030 \| Fluorescein-5-isothiocyanate (FITC) \| FT-0600599 \| 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)-5-isothiocyanatobenzoic acid \| FT-0626453 \| Fluorescein isothiocyanate iso
Specifications & Purity	10mM in DMSO
Biochemical and Physiological Mechanisms	Fluorescein isothiocyanate isomer I is an amine-reactive reagent for the FITC labeling of proteins, microsequencing of proteins, and peptides (HPLC).Fluorescent marker. Amine reactive fluorescein isomer. Yields conjugates with superior stability compared
Storage Temp	Protected from light,Store at -80°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	FITC can bind to various antibody proteins, and the bound antibody does not lose the specificity of binding to a certain antigen, and has strong yellow-green fluorescence in alkaline solution. The corresponding antigen can be qualitatively, localized or quantitatively detected by observation under a fluorescence microscope or flow cytometry analysis. Used in medicine, agriculture and animal husbandry, it can quickly diagnose diseases caused by bacteria, viruses and parasites. FITC labeled antibody process (1) The protein to be crosslinked (concentration ≥1mg/mL) was dialyzed three times against the crosslinking reaction solution at 4°C to pH=9.0. The preparation method of the crosslinking reaction solution: 7.56g NaHCO3, 1.06g Na2CO3, 7.36g NaCl, add water to make the volume to 1 L. (2) Dissolve FITC in DMSO at a concentration of 1 mg/mL. FITC used for each cross-linking should be freshly prepared and protected from light. (3) Slowly add FITC to the antibody solution at the ratio of P:F (Protein: FITC) = 1mg: 150μg, and gently shake to mix it with the antibody while adding, and react at 4°C in the dark for 8 hours. (4) Add 5mol/L NH4Cl to a final concentration of 50mmol/L, and terminate the reaction at 4°C for 2h. (5) Dialyze the cross-linked substance in PBS for more than four times until the dialysate is clear. (6) Identification of cross-linked products Protein concentration (mg/mL) = [A280– 0.31×A495] / 1.4 F/P ratio: 3.1×A495/ [A280 – 0.31×A495], the value should be between 2.5 and 6.5. (7) FITC cross-linked protein should be placed in a pH 7.4 phosphate buffer, added with 0.1% NaN3, 1% BSA, and stored at 4°C in the dark. application： hematology: suitable；histology: suitable

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Benzopyrans
    - Subclass 1-benzopyrans
      - Level5 Dibenzopyrans
        Level6 Xanthenes

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Benzopyrans
Subclass	1-benzopyrans
Intermediate Tree Nodes	Dibenzopyrans
Direct Parent	Xanthenes
Alternative Parents	Diarylethers Phthalides Benzofuranones Isobenzofurans 1-hydroxy-2-unsubstituted benzenoids Lactones Isothiocyanates Carboxylic acid esters Propargyl-type 1,3-dipolar organic compounds Oxacyclic compounds Monocarboxylic acids and derivatives Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isobenzofuran - Isocoumaran - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Carboxylic acid ester - Isothiocyanate - Lactone - Carboxylic acid derivative - Ether - Oxacycle - Organic 1,3-dipolar compound - Monocarboxylic acid or derivatives - Propargyl-type 1,3-dipolar organic compound - Hydrocarbon derivative - Organic nitrogen compound - Organonitrogen compound - Organooxygen compound - Organosulfur compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors	fluorescein isothiocyanate
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Names and Identifiers

IUPAC Name	3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
INCHI	InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChIKey	MHMNJMPURVTYEJ-UHFFFAOYSA-N
Smiles	C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
Isomeric SMILES	C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
WGK Germany	3
Molecular Weight	389.38
Beilstein	1407295
Reaxy-Rn	1407295
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1407295&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Chemical and Physical Properties

Sensitivity	Light，heat and moisture sensitive
Boil Point(°C)	~708.6 °C at 760 mmHg
Melt Point(°C)	>360°C
Molecular Weight	389.400 g/mol
XLogP3	4.800
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	1
Exact Mass	389.036 Da
Monoisotopic Mass	389.036 Da
Topological Polar Surface Area	120.000 Å²
Heavy Atom Count	28
Formal Charge	0
Complexity	668.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Documents & Articles

Cytoskeletal Staining Reagents

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5-FITC - 10mM in DMSO, high purity , CAS No.3326-32-7