首页
分子生物学
逆转录产品
UltraBio™ Hotstart HiTaq& Super M-MuLV One Step RT-qPCR Kit (UDG)

UltraBio™ Hotstart HiTaq& Super M-MuLV One Step RT-qPCR Kit (UDG)

级别和纯度:

for DNA and RNA applications

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
U1492590-100T	100T	期货
U1492590-1000T	1000T	期货
U1492590-10000T	10000T	期货

查看相关系列

Master Mix (10) PCR (26) RT-qPCR (9) 分子生物学用酶 (56)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

别名

反转录实时荧光定量PCR

英文别名

One Step RT-qPCR Master Mix | One-Step qRT-PCR | Real-time RT-PCR

规格或纯度

BioReagent, 无DNA酶和RNA酶, PCR试剂, 分子生物学级, for DNA and RNA applications

稳定性与储存

Store at -20℃ long term (24 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

英文名称

UltraBio™ Hotstart HiTaq& Super M-MuLV One Step RT-qPCR Kit (UDG)

储存温度

-20°C储存,避免反复冻融

运输条件

超低温冰袋运输

产品介绍

Hotstart HiTaq& Super M-MuLV One Step RT-qPCR Kit (with UDG) 以其卓越的快速性及定量性被广泛应用，使用该产品进行Real Time RT-PCR反应可在同一反应管内连续进行，操作简单。酶系使用热敏型的UDG酶，搭配含U的底物，可以有效防止上一次的PCR产物对本次实验额污染，同时热敏型的UDG酶在50℃的条件下，完全失去活性，不影响本次实验的逆转录产物。
本试剂中使用了稳定、高效的反转录酶（Super M-MuLV Reverse Transcriptase）和化学修饰热启动酶（Hotstart HiTaq DNA Polymerase），具有高扩增效率和高扩增特异性，能进行稳定的Real Time One Step RT-PCR反应，大大提高了检测灵敏度，并省略了PCR反应后的电泳步骤，非常适合于微量RNA的检测。
本试剂专为进行便利、高灵敏度的一步式RT-PCR设计。其独特的酶和特别设计的缓冲液确保了逆转录和PCR反应高效、准确的进行，无需进行优化。可以在宽广的定量区域内得到良好的标准曲线，对靶基因进行准确定量检测，重复性好，可信度高。

适用范围：以 RNA 为模板的核酸定量或定性检测，逆转录+扩增在一管内完成，无需开盖。

组分表

U1492590	Component	100T	1000T	10000T	Storage
U1492590A	Hotstart HiTaq& Super M-MuLV Mix	0.2 mL	2 mL	20 mL	-20℃
U1492590B	Buffer Mix	0.95 mL	9.5 mL	95 mL	-20℃

使用说明：
1、反应体系配制：
溶解并混匀 PCR 反应所需的各种溶液，瞬时离心后，放置室温待用，探针建议避光处理。建议 PCR 液体分装使用，避免反复冻融。
2、参照下表配制 PCR 反应体系：

组分	加入体积/25μL 体系	加入体积/50μL 体系	终浓度
Buffer mix	9.5 μL	19 μL	1×
酶系（Hotstart HiTaq& Super M-MuLV）	2μL	4 μL	2/50 μL
引物探针	1 μL	2 μL	/
模板	5 μL	10 μL	/
ddH2O	To 25 μL	To 50 μL	/
终体积	25 μL	50 μL

注：引物、探针以及模板的用量可自行调整。
3、用移液器轻轻吹打混匀或轻微 Vortex 混匀，室温离心数秒，使液体积聚于管底。
4、将各 PCR 反应管置于 PCR 仪上，开始 PCR 反应。

步骤名称	温度	时间	循环
逆转录	50℃	15min	1
预变性	95℃	10min	1
变性	95℃	10s	45
退火	60℃	40s	45
保存	12℃	∞	1

注：反应条件可根据实际情况自行调整。

Hotstart HiTaq& Super M-MuLV One Step RT-qPCR Kit (with UDG) is widely recognized for its exceptional speed and quantitative accuracy. This product enables continuous Real-Time RT-PCR reactions within a single tube, simplifying operations. The enzyme system employs thermolabile UDG (Uracil-DNA Glycosylase) in combination with dUTP-containing substrates, effectively preventing carryover contamination from previous PCR products. The thermolabile UDG is completely inactivated at 50°C, ensuring it does not interfere with the reverse transcription products of the current reaction.

This kit utilizes a highly stable and efficient reverse transcriptase (Super M-MuLV Reverse Transcriptase) and a chemically modified hot-start DNA polymerase (Hotstart HiTaq DNA Polymerase). It delivers high amplification efficiency and specificity, enabling robust Real-Time One-Step RT-PCR. This significantly enhances detection sensitivity and eliminates the need for post-PCR electrophoresis, making it particularly suitable for the detection of trace amounts of RNA.

Specifically designed for convenient and highly sensitive one-step RT-PCR, this kit features a unique enzyme formulation and specially optimized buffer system. These ensure efficient and accurate reverse transcription and PCR amplification without the need for reaction optimization. It generates excellent standard curves over a wide quantitative range, allowing precise quantitative detection of target genes with high reproducibility and reliability.

Applications: Quantitative or qualitative detection of nucleic acids using RNA as template. Reverse transcription and amplification are completed in a single tube without tube opening.

Product Component List

U1492590	Component	100T	1000T	10000T	Storage
U1492590A	Hotstart HiTaq& Super M-MuLV Mix	0.2 mL	2 mL	20 mL	-20℃
U1492590B	Buffer Mix	0.95 mL	9.5 mL	95 mL	-20℃

Protocol:

1. Reaction Setup:

Thaw and thoroughly mix all PCR solutions required for the reaction.
Briefly centrifuge the solutions and keep them at room temperature until use.
Probes should be protected from light.
Recommendation: Aliquot PCR reagents to avoid repeated freeze-thaw cycles.

2. Prepare the PCR Reaction Mixture according to the table below:

Component	Volume/25 μL	Volume/50 μL	Final Concentration
Buffer Mix	9.5 μL	19 μL	1×
Enzyme Mix (Hotstart HiTaq& Super M-MuLV)	2 μL	4 μL	2/50 μL
Primer-Probe Mix	1 μL	2 μL	/
Template	5 μL	10 μL	/
ddH2O	To 25 μL	To 50 μL	/
Total Volume	25 μL	50 μL

Note: The amounts of primers, probes, and template can be adjusted according to experimental requirements.

3. Mixing:
Gently mix the reaction components by pipetting up and down or by brief vortexing. Centrifuge briefly at room temperature to collect the liquid at the bottom of the tube.

4. PCR Amplification:
Place the PCR reaction tubes in the thermal cycler and initiate the PCR program.

Standard Thermal Cycling Protocol:

Step	Temperature	Time	Cycles
Reverse Transcription	50℃	15min	1
Initial Denaturation	95℃	10min	1
Denaturation	95℃	10s	45
Annealing/Extension	60℃	40s	45
Hold	12℃	∞	1

Note: Reaction conditions can be adjusted based on specific experimental requirements.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度