| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| tl155918-200μg |
200μg |
期货  |
|
| 产品名称 | Mouse brain tissue lysate - total protein |
|---|---|
| 规格或纯度 | 200μg |
| 产品介绍 |
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE and WB analysis. The concentration was determined using the BCA assay process. |
| 应用 | WB |
|---|---|
| 物理形态 | 冻干(Lyophilized) |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80°C. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20°C for short-term storage or -80°C for long-term storage. |
| 分子类型 | 细胞或组织提取物 |
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: CAMK2B Mouse mAb (Ab092658) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 59 kDa
Exposure time: 49.2 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: ZnT-3 Antibody (Ab135449) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 6.6 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant PPP1CA/B Antibody (Ab122819) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 35 kDa
Exposure time: 36.5 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Quinone oxidoreductase Antibody (Ab124077) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 17.7 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Haptoglobin Antibody (Ab008379) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 39 kDa
Exposure time: 23.5 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: MSC Antibody (Ab116083) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 28 kDa
Exposure time: 1.8 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Otx1 Antibody (Ab119495) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 100.0 s
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