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TNT缓冲液 (10×)

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
T476217-500ml
500ml 现货 Stock Image

基本描述

英文别名 TRIS NaCl Tween 20 buffer solution | Tris-NaCl-Tween buffer | 10X TNT Buffer
规格或纯度 BioReagent, 无菌过滤, 分子生物学级, 10×
稳定性与储存 Store at room temperature long term (12 months).
英文名称 TNT Buffer (10×)
储存温度 室温
运输条件 常规运输
产品介绍

  Tris-NaCl-Tween buffer简称TNT,常用的分子生物学和杂交实验的缓冲液,主要由100mM Tris-HCl (pH8.0)、1.5M NaCl、0.5%Tween组成,经0.45μm孔径膜过滤,严格灭菌制备而成。本产品为10倍浓缩液,经过过滤灭菌处理,使用时只需要用无菌蒸馏水或去离子水稀释至工作浓度即可。

操作步骤:

1. 用蒸馏水或去离子水稀释到1×。

2. 按照实验需要洗涤使用。

注意事项:

1. 试剂开封后请尽快使用,以防影响后续实验效果。

2. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

3. 本产品不含剧毒,但为了您的安全和健康,请穿实验服并戴一次性手套操作。

  Tris-NaCl-Tween Buffer, abbreviated as TNT Buffer, is a commonly used buffer in molecular biology and hybridization experiments. It mainly consists of 100 mM Tris-HCl (pH 8.0), 1.5 M NaCl, and 0.5% Tween. The product is prepared through strict sterilization by filtration with a 0.45 μm pore size membrane. This product is a 10× concentrated solution; after filtration sterilization, it only needs to be diluted to the working concentration with sterile distilled water or deionized water before use.
Operating Steps:
1. Dilute to 1× with distilled water or deionized water.
2. Use the diluted buffer for washing as required by the experiment.
Precautions:
1. After opening the reagent, use it as soon as possible to avoid affecting the results of subsequent experiments.
2. This product is limited to scientific research use by professional personnel. It shall not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals, and shall not be stored in ordinary residential premises.
3. This product does not contain highly toxic substances; however, for your safety and health, please wear a lab coat and disposable gloves during operation.

名称和识别符

分子类型 生物试剂/缓冲液

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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批号(Lot Number) 证书类型 货号
ZJ25F1028459 分析证书 T476217

技术文档和文章

引用文献

1. D A Baunoch, P Das, M E Browning, V Hari,.  (1992-03-01)  R-ELISA: repeated use of antigen-coated plates for ELISA and its application for testing of antibodies to HIV and other pathogens..  BioTechniques,  12  ((3)): (412-417).  [PMID:1571153]

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