TPE缓冲液 (10×)

COA

级别和纯度:

BioReagent

用于核酸电泳

10×

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
T301544-500ml	500ml	现货

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Bioreagents/Buffer (3) NA electrophoresis (2)

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基本描述

英文别名	Tris-Phosphate-EDTA Buffer
规格或纯度	BioReagent, 用于核酸电泳, 10×
稳定性与储存	Store at room temperature long term (12 months).
英文名称	TPE Buffer (10×)
储存温度	室温
运输条件	常规运输
产品介绍	Tris-磷酸电泳缓冲液简称TPE，常用于RNA凝胶电泳，主要由900mM Tris-磷酸、20mM EDTA等组成。本产品为10×浓缩液，使用时需用蒸馏水或去离子水稀释为1×TPE后使用。操作步骤： 1. 用蒸馏水或去离子水稀释到1×。 2. 琼脂糖溶解于1×TPE，加热熔化，冷却至55℃后加染料，冷却至室温。 3. 上样，电泳。注意事项： 1. 试剂开封后请尽快使用，以防影响后续实验效果。 2. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 3. 本产品不含剧毒，但为了您的安全和健康，请穿实验服并戴一次性手套操作。 Tris-Phosphate Electrophoresis Buffer, abbreviated as TPE Buffer, is commonly used in RNA gel electrophoresis. It mainly consists of 900 mM Tris-phosphate, 20 mM EDTA, and other components. This product is a 10× concentrated solution and needs to be diluted to 1× TPE with distilled water or deionized water before use. Operating Steps: 1. Dilute to 1× with distilled water or deionized water. 2. Dissolve agarose in 1× TPE, heat to melt it. After cooling to 55°C, add the dye, then cool to room temperature. 3. Load the sample and perform electrophoresis. Precautions: 1. After opening the reagent, use it as soon as possible to avoid affecting the results of subsequent experiments. 2. This product is limited to scientific research use by professional personnel. It shall not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals, and shall not be stored in ordinary residential premises. 3. This product does not contain highly toxic substances; however, for your safety and health, please wear a lab coat and disposable gloves during operation.