基本描述

规格或纯度	BioReagent, 即用型, 无支原体, 分子生物学级, 无RNA酶, 无菌, for DNA and RNA applications
稳定性与储存	Store at Room temperature term (12 months). Upon receipt, it is recommended to aliquot.
英文名称	Sample Protector for RNA
可用pH范围	5.0-5.2
储存温度	室温
运输条件	常规运输
产品介绍	产品介绍：生物样本被收集后，其RNA开始变得相当不稳定。快速稳定RNA及保持RNA表达量是获得精确的基因表达分析数据的首要条件。此外，也需要防止由于样本处理引起的基因表达升高或下调的激活。样本RNA保护剂是一种液态无毒的动物组织保存试剂。它能迅速渗入组织细胞中, 通过高效的抑制RNase活性保护非冷冻细胞RNA，使其免受降解，从而使得在获得组织样品后，不必马上处理样本,也不必将样本冶冻在液氮之中,而更方便后续实验操作。使用组织RNA保护液，可免去使用液氮或超低温冰箱的不便，而且把不同批次的组织标本存放在该保护液中，能立即终止并固定RNA表达的时序变化，可以减少实验组间误差样本RNA保护剂可广泛应用于多种脊椎动物样本，包括脑、心、肾、脾、肝、肺。新鲜非冷冻组织按1:10比例浸入样本RNA保护剂后，样品可在37°C保存1天，常温保存1周，4°C保存1个月；组织4°C浸泡处理后可以在-20°C或-80°C长期保存。注意事项： 1. 样本RNA保护剂可能会产生沉淀或晶体析出，使用之前需室温或37°C完全溶解。 2. 样本RNA保护剂只适用于新鲜动物组织，不能用于冷冻组织。 3. 组织块的大小要求任何一边不能超过0.5cm ,若组织过大，可先将其剪碎后再浸泡于10倍体积的样本RNA保护剂中。 4. 保存于样本RNA保护剂中的组织如果需要长途运输,运输过程中需要确保组织完全浸入样本RNA保护剂中。 5. 为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。使用说明： 1 动物组织 1.1 切割组织前估计使用组织的体积(或者重量)，按1:10(组织:样本RNA保护剂)的比例取出样本RNA保护剂以便使用(例如：组织量100mg，需样本RNA保护剂1mL，等比缩小对实验结果无影响，等比放大需剪碎组织。)若组织样本过大需剪切边长小于0.5cmx0.5cmx0.5cm的组织块后再进行保存。在保存时应注意组织块必须完全浸入保护液中。注意:样本RNA保护剂的用量至少10倍于组织的体积(或重量)。 1.2 长期储存可置于-80°C ：将样本与样本RNA保护剂一起在2~8°C过夜孵育，然后将样本取出，并保存于-80°C。对于培养的细胞，可直接将含有细胞的样本RNA保护剂冻存。样本使用时，在室温下融化。 1.3 长期储存也可置于-20°C：将样本与样本RNA保护剂一起在2~8°C过夜孵育，然后转移至-20°C。浸泡于样本RNA保护剂中的样本在-20°C可能不会结冻。低温保存会使溶液形成结晶或沉淀，这不会影响RNA的保护效果以及后续RNA的纯化。如果担心结晶会影响后续实验，可在冷冻前, 将样本取出, 然后冻存。 1.4 短期储存于2~8°C：将样本与样本RNA保护剂-起在2~8°C孵育，可储存一月。 2 植物组织可將植物组织浸泡于10倍体积的样本RNA保护剂中。因植物组织的复杂性，并不一定适合于所有的组织。植物组织有天然屏障，阻止扩散，例如，叶片表面的蜡质，需要将其破坏，使样本RNA保护剂渗透入组织中。任何破坏蜡质的方法都可以使用，例如切割或物理撕裂。储存方法见上。 3 培养细胞用实验室标准流程沉淀细胞。用PBS进行洗涤，去除培养基。用小量PBS重悬细胞，加入10倍体积的样本RNA保护剂。储存方法见上。 4 白细胞从全血中分离白细胞后，按照“培养细胞"的方法加入样本RNA保护剂，可保存白细胞。未从全血中分离的白细胞不能用样本RNA保护剂保存。因为其中含有高浓度的蛋白，加入样本RNA保护剂后会形成沉淀。储存方法见上。 5 细菌按照“培养细胞"的方法加入样本RNA保护剂，可保存大肠杆菌中的RNA。储存方法见上。 6 后续分离RNA实验 6.1 对于组织样本，可用无菌镊子将样本从样本RNA保护剂中取出，浸泡于RNA分离的裂解液中。对于组织，应快速均浆。注意:将组织储存于样本RNA保护剂中,会使其变得坚硬，与新鲜组织相比,均浆会更加困难一点儿。用解剖刀将组织切割为小块，会使均浆容易一些。 6.2 对于细胞样本，可用离心的方法收集细胞，去除样本RNA保护剂；也可直接从混合物中提取RNA。由于样本RNA保护剂比细胞培养基密度大,在常规离心力下无法使细胞沉淀。例如, 对于HeLa细胞，3000g可使细胞沉淀。 6.3 如果使用一步法抽提RNA，例如使用Trizol，在抽提的过程中，水相可能会变得模糊，类似于云雾状，不影响RNA质量，可继续按照Trizol的说明书抽提RNA。 Product Introduction: Once a biological sample is collected, its RNA begins to become quite unstable. Rapid stabilization of RNA and maintenance of RNA expression levels are the primary conditions for obtaining accurate gene expression analysis data. In addition, it is necessary to prevent the activation of gene expression elevation or downregulation caused by sample processing. Sample RNA protectant is a liquid non-toxic animal tissue preservation reagent. It can quickly penetrate into tissue cells and protect non frozen cell RNA from degradation by efficiently inhibiting RNase activity, making it easier to process and freeze tissue samples in liquid nitrogen after obtaining them, and facilitating subsequent experimental operations. The use of tissue RNA protective solution eliminates the inconvenience of using liquid nitrogen or ultra-low temperature refrigerators. Moreover, storing different batches of tissue specimens in this protective solution can immediately terminate and fix the temporal changes in RNA expression, reducing experimental group errors. Sample RNA protective agents can be widely used in various vertebrate samples, including brain, heart, kidney, spleen, liver, and lung. After immersing fresh non frozen tissue in sample RNA protectant at a ratio of 1:10, the sample can be stored at 37 °C for 1 day, room temperature for 1 week, and 4 °C for 1 month; After soaking at 4 °C, the tissue can be stored for a long time at -20 °C or -80 °C. Precautions： 1. Sample RNA protectants may precipitate or crystallize, and should be completely dissolved at room temperature or 37 ° C before use. 2. Sample RNA protectants are only suitable for fresh animal tissues and cannot be used for frozen tissues. 3. The size of the tissue block should not exceed 0.5cm on any side. If the tissue is too large, it can be cut into pieces and then soaked in 10 times the volume of sample RNA protectant. If the tissue stored in the sample RNA protectant needs to be transported over long distances, it is necessary to ensure that the tissue is completely immersed in the sample RNA protectant during transportation. 5. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents. Instructions for Use： 1 Animal organization 1.1 Before cutting the tissue, estimate the volume (or weight) of the tissue to be used. Take out the sample RNA protectant at a ratio of 1:10 (tissue: sample RNA protectant) for use (for example, if the tissue volume is 100mg, 1mL of sample RNA protectant is required. Proportional reduction has no effect on the experimental results, and proportional enlargement requires cutting the tissue. If the tissue sample is too large, tissue blocks with a side length less than 0.5cmx0.5cmx0.5cm should be cut before storage. When storing, it should be noted that the tissue block must be completely immersed in the protective solution. Note: The amount of sample RNA protectant should be at least 10 times the volume (or weight) of the tissue. 1.2 Long term storage can be placed at -80 ° C: Incubate the sample with sample RNA protectant overnight at 2-8 ° C, then remove the sample and store it at -80 ° C. For cultured cells, the sample RNA protectant containing cells can be directly frozen. When using the sample, melt it at room temperature. 1.3 Long term storage can also be placed at -20 ° C: Incubate the sample with sample RNA protectant overnight at 2-8 ° C, and then transfer to -20 ° C. Samples soaked in RNA protectants may not freeze at -20 ° C. Low temperature storage will cause crystallization or precipitation of the solution, which will not affect the protective effect of RNA and subsequent purification of RNA. If you are concerned that crystallization may affect subsequent experiments, you can remove the sample before freezing and then freeze it. 1.4 Short term storage at 2-8 ° C: Incubate the sample with sample RNA protectant at 2-8 ° C and store for one month. 2 Plant tissues Plant tissue can be soaked in 10 times the volume of sample RNA protectant. Due to the complexity of plant tissue, it may not be suitable for all tissues. Plant tissues have natural barriers that prevent diffusion, such as the wax on the surface of leaves, which needs to be broken down to allow the sample RNA protectant to penetrate into the tissue. Any method of breaking wax can be used, such as cutting or physical tearing. See the storage method above. 3. Cultivate cells Use laboratory standard procedures to precipitate cells. Wash with PBS to remove the culture medium. Resuspend cells with a small amount of PBS and add 10 times the volume of sample RNA protectant. See the storage method above. 4 white blood cells After isolating white blood cells from whole blood, adding sample RNA protectant according to the method of "culturing cells" can preserve white blood cells. White blood cells that have not been isolated from whole blood cannot be preserved with sample RNA protectant because they contain high concentrations of protein, which will form a precipitate after adding sample RNA protectant. Storage methods are described above. 5 bacteria Adding sample RNA protectant according to the method of "cultivating cells" can preserve RNA in Escherichia coli. The storage method is shown above. 6 Subsequent RNA isolation experiments 6.1 For tissue samples, sterile forceps can be used to remove the sample from the sample RNA protectant and soak it in the RNA separation lysis buffer. For organizations, rapid homogenization is necessary. Attention: Storing the tissue in a sample RNA protectant will make it harder and more difficult to homogenize compared to fresh tissue. Using a dissecting knife to cut tissue into small pieces will make homogenization easier. 6.2 For cell samples, centrifugation can be used to collect cells and remove sample RNA protectants; RNA can also be extracted directly from the mixture. Due to the higher density of sample RNA protectants compared to cell culture media, cells cannot be precipitated under conventional centrifugal force. For example, for HeLa cells, 3000g can induce cell precipitation. 6.3 If a one-step method is used to extract RNA, such as using Trizol, the aqueous phase may become blurry during the extraction process, resembling a cloud like state, which does not affect the quality of RNA. RNA extraction can continue according to Trizol's instructions.

名称和识别符

分子类型	生物试剂/缓冲液

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批号(Lot Number)	证书类型	货号
ZJ25F0521263	分析证书	S766791
ZJ25F0421088	分析证书	S766791

技术文档和文章

s766791_样品保存稳定剂产品说明书.pdf

s766791_ sample protector for rna product manual.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

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分子量

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稀释计算器

计算配制储备溶液所需的稀释度。

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浓度 2 (C2)

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体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

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所需的复溶浓度

货号 (SKU)	包装规格	是否现货	价格	数量
S766791-100ml	100ml	期货
S766791-500ml	500ml	期货

样品保存稳定剂

库存信息