| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| S292620-1ml |
1ml |
现货  |
|
| 产品名称 | SA-HRP（HRP 标记的链霉亲和素） | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 规格或纯度 | 500U/ml | ||||||||||||||||||||
| 产品介绍 |
产品说明 SA-HRP (HRP-labeled Streptavidin)中文名为辣根过氧化物酶标记Streptavidin也称辣根过氧化物酶标记链霉亲和素。本HRP-Streptavidin用高纯度的Streptavidin和超纯的辣根过氧化物酶交联而成，确保产生最低的本底和最高的灵敏度。 Streptavidin的分子量为60kD，可以高度特异性地和生物素(Biotin)结合。Streptavidin和生物素的亲和常数为Kd=10-15M。 Streptavidin是一个四聚体蛋白，可以同时结合4个生物素分子。Streptavidin的中文名为链霉亲和素，为重组表达的，和鸡蛋清来源的Avidin(亲和素)在空间结构以及与生物素的亲和力方面具有 高度的相似性。和Avidin不同的是，Streptavidin是一种非糖基化蛋白，并且基本不带电荷。Avidin的pI= ~10.5，在中性pH条件下呈碱性。由于Streptavidin和Avidin相比在中性条件下不带电荷，因此Streptavidin的非特异性结合比Avidin低很多，这样检测时的非特异性背景就低很多。因此目前在生物素检测时通常使用Streptavidin替代Avidin。 辣根过氧化物酶即horseradish peroxidase (HRP)，可以在Western、EMSA、Southern或Northern等检测时催化ECL类试剂(如 ECL、BeyoECL等)产生化学发光，可以在ELISA时催化TMB产生蓝色，也可以在免疫组化、免疫细胞化学或Western blot 检测时催化DAB产生棕色沉淀。
可以用于生物素(Biotin)标记的抗体、核酸、蛋白或其它生物素标记分子的检测。具体用途包括 Western、ELISA、免疫组化或免疫细胞化学、原位杂交、Southern、Northern、EMSA 等。
本辣根过氧化物酶标记Streptavidin 用于各种常规用途的推荐稀释比例参考下表，实际实验操作过程中需根据具体情况适当调节辣根过氧化物酶标记 Streptavidin 的稀释比例。对于 Western，推荐的稀释范围为1:2000-10000。
HC，Immunohistochemistry；IC，Immunocytochemistry；ISH，in situ hybridization.
叠氮钠会抑制辣根过氧化物酶的活性，在含有辣根过氧化物酶的溶液中不可加入叠氮钠。为了您的安全和健康，请穿实验服并戴一次性手套操作。
Western、ELISA、免疫组化或免疫细胞化学、原位杂交、Southern、Northern、EMSA 等请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。 Product manual SA-HRP (HRP-labeled Streptavidin) Chinese name is horseradish peroxidase-labeled Streptavidin, also known as horseradish peroxidase-labeled streptavidin. The HRP-Streptavidin is cross-linked with high-purity Streptavidin and ultra-pure horseradish peroxidase to ensure the lowest background and highest sensitivity. Streptavidin has a molecular weight of 60kD and can bind to Biotin with a high degree of specificity. The affinity constant of Streptavidin and biotin is Kd=10-15M. Streptavidin is a tetrameric protein that can bind 4 biotin molecules at the same time. Streptavidin's Chinese name is streptavidin. It is recombinantly expressed and has a high degree of similarity with Avidin from egg white in terms of spatial structure and affinity with biotin. Unlike Avidin, Streptavidin is a non-glycosylated protein and is basically uncharged. Avidin has a pI= ~10.5, which is alkaline under neutral pH conditions. Since Streptavidin has no charge under neutral conditions compared to Avidin, the non-specific binding of Streptavidin is much lower than that of Avidin, so the non-specific background during detection is much lower. Therefore, Streptavidin is usually used instead of Avidin in biotin testing. Horseradish peroxidase, namely horseradish peroxidase (HRP), can catalyze ECL reagents (such as ECL, BeyoECL, etc.) to produce chemiluminescence during Western, EMSA, Southern or Northern detection, and can catalyze TMB to produce blue color during ELISA. It can also catalyze DAB to produce brown precipitates in immunohistochemistry, immunocytochemistry or Western blot detection.
It can be used for the detection of Biotin-labeled antibodies, nucleic acids, proteins or other biotin-labeled molecules. Specific uses include Western, ELISA, immunohistochemistry or immunocytochemistry, in situ hybridization, Southern, Northern, EMSA, etc.
Refer to the table below for the recommended dilution ratio of horseradish peroxidase-labeled Streptavidin for various general purposes. In the actual experimental operation, the dilution ratio of horseradish peroxidase-labeled Streptavidin should be appropriately adjusted according to the specific conditions. For Western, the recommended dilution range is 1:2000-10000.
HC，Immunohistochemistry；IC，Immunocytochemistry；ISH，in situ hybridization.
Sodium azide can inhibit the activity of horseradish peroxidase. Sodium azide should not be added to the solution containing horseradish peroxidase. For your safety and health, please wear lab coats and disposable gloves for operation.
For Western, ELISA, immunohistochemistry or immunocytochemistry, in situ hybridization, Southern, Northern, EMSA, etc., please refer to the relevant experimental procedures. The initial dilution concentration is diluted according to the recommended dilution ratio in the product introduction. |
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| 偶联 | HRP |
| 浓度 | 500U/ml |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 分子类型 | 未知 |
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