Recombinant Human CFTR Protein

COA

级别和纯度:

无载体

≥95%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P13569
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp218615-10μg	10μg	现货
rp218615-50μg	50μg	现货
rp218615-100μg	100μg	现货
rp218615-1mg	1mg	期货

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Accession#: P13569 (1) CFTR (52) Gene ID: 1080 (1)

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基本描述

产品名称	Recombinant Human CFTR Protein
别名	重组人CFTR蛋白 \| 重组人囊性纤维化跨膜调节因子蛋白
英文别名	ABC 35 \| ABC35 \| ABCC 7 \| ABCC7 \| ATP binding cassette sub family C member 7 \| ATP Binding Cassette Superfamily C Member 7 \| ATP binding cassette transporter sub family C member 7 \| ATP-binding cassette sub-family C member 7 \| cAMP dependent chloride chan
规格或纯度	无载体, ≥95%(SDS-PAGE), 见COA
生化机理	Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed:26823428). Mediates the transport of chloride ions across the cell membrane (PubMed:10792060, PubMed:11524016, PubMed:11707463, PubMed:12519745, PubMed:12529365, PubMed:12588899, PubMed:12727866, PubMed:15010471, PubMed:17036051, PubMed:1712898, PubMed:17182731, PubMed:19398555, PubMed:19621064, PubMed:22178883, PubMed:25330774, PubMed:26846474, PubMed:28087700, PubMed:8910473, PubMed:9804160). Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains (PubMed:11524016, PubMed:15284228, PubMed:26627831, PubMed:8910473). The ion channel is also permeable to HCO3-; selectivity depends on the extracellular chloride concentration (PubMed:15010471, PubMed:19019741). In vitro, mediates ATP-dependent glutathione flux (PubMed:12727866). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed:12403779, PubMed:22121115, PubMed:22178883, PubMed:27941075). Plays an important role in airway fluid homeostasis (PubMed:16645176, PubMed:19621064, PubMed:26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed:14668433, PubMed:16645176, PubMed:26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed:17182731, PubMed:17434346, PubMed:27941075). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed:17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed:17182731, PubMed:27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed:12403779). Can inhibit the chloride channel activity of ANO1 (PubMed:22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed:19923167, PubMed:27714810). Post-translational: N-glycosylated. hosphorylated; cAMP treatment promotes phosphorylation and activates the channel (PubMed:12588899, PubMed:17036051, PubMed:8910473). Dephosphorylation decreases the ATPase activity (in vitro) (PubMed:8910473). Phosphorylation at PKA sites activates the channel (PubMed:10792060, PubMed:12519745, PubMed:12588899, PubMed:25330774). Phosphorylation at PKC sites enhances the response to phosphorylation by PKA (PubMed:12588899). Phosphorylated by AMPK; this inhibits channel activity (PubMed:12519745). Ubiquitinated, leading to its degradation in the lysosome (PubMed:19398555, PubMed:23818989). Deubiquitination by USP10 in early endosomes enhances its endocytic recycling to the cell membrane (PubMed:19398555). Ubiquitinated by RNF185 during ER stress (PubMed:24019521). Ubiquitinated by MARCHF2 (PubMed:23818989).
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	1377-1480 aa
序列	MHHHHHHDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKSKPQIAALKEETEEEVQDTRL
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P13569
来源	重组表达
预测分子量	13.2 kDa
SDS-PAGE	15.2 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 500M NaCl, 8M Urea, 250mM Imidazole, pH 8.0.
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃保存 12个月；复溶后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human CFTR Protein (rp218615)

Recombinant Human CFTR Protein (rp218615) - SDS-PAGE
3 μg/lane of Recombinant Human CFTR Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 15.2 kDa under reducing conditions.

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批号(Lot Number)	证书类型	货号
ZJ25F0622956	分析证书	rp218615
ZJ25F0622955	分析证书	rp218615
ZJ25F0622954	分析证书	rp218615

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