Recombinant Human ALK/CD246 Protein

COA

级别和纯度:

无载体

≥85%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: Q9UM73
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp217142-10μg	10μg	现货
rp217142-50μg	50μg	现货
rp217142-100μg	100μg	现货
rp217142-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: ALK

Accession#: Q9UM73 (2) ALK (2) ALK/CD24 (1) Gene ID: 238 (2)

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基本描述

产品名称	Recombinant Human ALK/CD246 Protein
别名	重组人ALK/CD246蛋白 \| 重组人退行性淋巴瘤激酶
英文别名	ALK tyrosine kinase receptor \| ALK \| anaplastic lymphoma kinase (Ki-1) \| Anaplastic lymphoma kinase \| anaplastic lymphoma receptor tyrosine kinase \| CD246 antigen \| CD246 \| EC 2.7.10.1 \| Ki-1 \| NBLST3 \| Tcrz
规格或纯度	无载体, ≥85%(SDS-PAGE), 见COA
生化机理	Anaplastic Lymphoma Kinase (ALK), also known as CD246, is a transmembrane receptor tyrosine kinase in the Insulin Receptor family. The ALK gene is a target of multiple chromosomal translocations in cancer that encode hybrid proteins which promote cellular transformation. Human ALK consists of a 1020 amino acid (aa) extracellular domain (ECD) with two MAM domains that flank an LDLR class A domain, a 21 aa transmembrane segment, and a 561 aa cytoplasmic domain that contains the tyrosine kinase domain. Within the ECD, human ALK shares 89% aa sequence identity with mouse and rat ALK. It is primarily expressed in the developing nervous system but is also found in various non‑neural tissues. Mature ALK is expressed on the cell surface as a 200 ‑ 220 kDa N‑glycosylated protein. Proteolytic cleavage of ALK liberates an 80 kDa soluble fragment from the ECD with a 140 kDa fragment remaining cell‑associated. ALK is classified as a dependence receptor, a protein that promotes apoptosis in the absence of ligand but is anti‑apoptotic upon stimulation. Its cytoplasmic domain is cleaved following Asp1160 by Caspases during apoptosis. ALK stimulation by antibody ligation induces activation of its kinase domain and receptor phosphorylation, enabling the association of ALK with signal transduction proteins. ALK binds the cytokines Pleiotrophin and Midkine, although their effects on cellular responses are not consistent between different systems. ALK promotes neurite formation in neuroblastoma cells and mediates the neuroprotective effects of Pleiotrophin in motor neurons. A t(2;5) chromosomal translocation with Nucleophosmin (NPM) in human anaplastic large cell lymphoma results in a hybrid protein consisting of NPM fused to the kinase domain of ALK. NPM‑ALK as well as other ALK fusion proteins are aberrantly localized to the cytoplasm and nucleus, have constitutively active kinase domains, and promote cellular transformation. Post-translational: Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity (PubMed:11121404, PubMed:15938644, PubMed:16878150, PubMed:34819673). In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation (PubMed:17681947). Phosphorylation at Tyr-1507 is critical for SHC1 association (PubMed:17274988).N-glycosylated.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	1360-1530 aa
序列	MHHHHHHRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDR
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	Q9UM73
来源	重组表达
预测分子量	20.0 kDa
SDS-PAGE	25.6 kDa, under reducing conditions; 25.6 kDa, under non-reducing conditions.

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 500mM NaCl, 1mM DTT, 5% Glycerol, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃ 存储12个月；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human ALK/CD246 Protein (rp217142)

Recombinant Human ALK/CD246 Protein (rp217142) - SDS-PAGE
3 μg/lane of Recombinant Human ALK/CD246 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 25.6 kDa.

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批号(Lot Number)	证书类型	货号
ZJ25F0926529	分析证书	rp217142
ZJ25F0926528	分析证书	rp217142
ZJ25F0926527	分析证书	rp217142

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