Recombinant Human CXCR4 Protein

COA

级别和纯度:

无载体

≥90%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P61073
蛋白标签: No tag
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp214408-10μg	10μg	现货
rp214408-50μg	50μg	现货
rp214408-100μg	100μg	现货
rp214408-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CXCR4

Accession#:P61073 (7) Cxcr4 (7) Gene ID:7852 (7)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human CXCR4 Protein
别名	重组人CXCR4 蛋白 \| 重组人C-X-C趋化因子受体4型蛋白
英文别名	C-X-C chemokine receptor type 4 \| CD184 \| CD184 antigen \| Chemokine (C X C motif) receptor 4 \| Chemokine CXC Motif Receptor 4 \| CXC-R4 \| CXCR-4 \| CXCR4 \| CXCR4_HUMAN \| D2S201E \| FB22 \| Fusin \| HM89 \| HSY3RR \| LAP 3 \| LAP3 \| LCR1 \| LESTR \| Leukocyte derive
规格或纯度	无载体, ≥90%(SDS-PAGE), 见COA
生化机理	Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Post-translational: Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.Ubiquitinated after ligand binding, leading to its degradation (PubMed:28978524). Ubiquitinated by ITCH at the cell membrane on agonist stimulation (PubMed:14602072, PubMed:34927784). The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5SSulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization.O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-38 aa
序列	GMEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNK
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	P61073
来源	重组表达
预测分子量	4.5 kDa
SDS-PAGE	16.2 kDa, under reducing conditions; 15.2 & 18.8 kDa, under non-reducing conditions.

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 100mM NaCl, 5% Glycerol, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃ 存储12个月；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human CXCR4 Protein (rp214408)

Recombinant Human CXCR4 Protein (rp214408) - SDS-PAGE
3 μg/lane of Recombinant Human CXCR4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.2 kDa under reducing conditions and 15.2 & 18.8 kDa under non-reducing conditions.
CXCR4 can form homodimers, which are formed through hydrophobic interactions between transmembrane domains under non-reducing conditions (PubMed: 20929726).

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927855	分析证书	rp214408
ZJ25F0927854	分析证书	rp214408
ZJ25F0927853	分析证书	rp214408

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度