Recombinant Human KRAS (G13D) Protein

COA

级别和纯度:

无载体

≥95%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P01116-2
蛋白标签: N-His
生物活性: The specific activity of KRAS was determined to be >3nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp192047-10μg	10μg	期货
rp192047-50μg	50μg	期货
rp192047-100μg	100μg	期货
rp192047-1mg	1mg	期货

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点击查看蛋白信息: KRAS

Accession#: P01116-2 (11) Gene ID: 3845 (12) KRAS (55) KRAS (G13D) (1)

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基本描述

产品名称	Recombinant Human KRAS (G13D) Protein
别名	重组人KRAS (G13D)蛋白 \| 重组人Kirsten大鼠肉瘤病毒癌基因同源物(G12S)蛋白
英文别名	C-K-RAS Protein \| c-Ki-ras Protein \| c-Ki-ras2 Protein \| CFC2 Protein \| K-Ras Protein \| K-Ras 2 Protein \| K-RAS2A Protein \| K-RAS2B Protein \| K-RAS4A Protein \| K-RAS4B Protein \| KI-RAS Protein \| KRAS1 Protein \| KRAS2 Protein \| NS Protein \| NS3 Protein \| O
规格或纯度	无载体, ≥95%(SDS-PAGE), 见COA
生化机理	K-Ras belongs to the small GTPase superfamily, Ras family. Like other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example, RasGAP. In turn, K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example, SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations are associated with Noonan syndrome and Cardio-Facio-Cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer, and lung cancer. Post-translational: Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
生物活性	The specific activity of KRAS was determined to be >3nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-185 aa (G13D)
序列	MHHHHHHTEYKLVVVGAGDVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P01116-2
来源	重组表达
预测分子量	21.9 kDa
SDS-PAGE	21.0 kDa, under reducing conditions; 21.0 kDa, under non-reducing conditions

储存与运输

物理形态	液体
储存缓冲液	25mM Tris, 100mM Glycine, 10% Glycerol, pH 7.5.
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

Enzyme Activity: Recombinant Human KRAS(G13D) Protein (rp192047)

Recombinant Human KRAS(G13D) Protein - Enzyme Activity
The specific activity of KRAS was determined to be >3.0 nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.

SDS-PAGE: Recombinant Human KRAS(G13D) Protein (rp192047)

Recombinant Human KRAS(G13D) Protein (rp192047) - SDS-PAGE
3 μg/lane of Recombinant Human KRAS(G13D) Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.0 kDa.

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