Recombinant Human TrkA Protein

COA

级别和纯度:

≥95%(SDS-PAGE)
见COA

表达系统: HEK293
Accession #: P04629
蛋白标签: C-His
生物活性: Testing in progress
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp189427-10μg	10μg	现货
rp189427-50μg	50μg	现货
rp189427-100μg	100μg	现货
rp189427-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: NTRK1

Accession#:P04629 (4) Gene ID:4914 (4) NTRK1 (4) TrkA (4)

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基本描述

产品名称	Recombinant Human TrkA Protein
别名	重组人TrkA蛋白 \| 重组人酪氨酸激酶受体A蛋白
英文别名	DKFZp781I14186 \| EC 2.7.10 \| EC 2.7.10.1 \| MTChigh affinity nerve growth factor receptor \| Neurotrophic tyrosine kinase receptor type 1 \| neurotrophic tyrosine kinase, receptor, type 1 \| NTRK1 \| NTRK-1 \| p140-TrkA \| TRK1-transforming tyrosine kinase prote
规格或纯度	≥95%(SDS-PAGE), 见COA
生化机理	TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by the pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation, and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma. Post-translational: Ligand-mediated autophosphorylation (PubMed:1281417, PubMed:15488758, PubMed:27676246, PubMed:28177573, PubMed:2927393, PubMed:7510697, PubMed:8155326, PubMed:8325889). Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1 (PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889). N-glycosylated (PubMed:2927393). Isoform TrkA-I and isoform TrkA-II are N-glycosylated. Ubiquitinated (PubMed:27445338). Undergoes polyubiquitination upon activation; regulated by NGFR (PubMed:27445338). Ubiquitination by NEDD4L leads to degradation (PubMed:27445338). Ubiquitination regulates the internalization of the receptor (By similarity).
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-423 aa
序列	MLRGGRRGQLGWHSWAAGPGSLLAWLILASAGAAPCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSGDPVEKKDETPFGVSVAVGHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P04629
来源	重组表达
预测分子量	43.2 kDa
SDS-PAGE	56.6-89.3 kDa, under reducing conditions; 54.2-84.1 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4.
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human TrkA Protein (rp189427)

Recombinant Human TrkA Protein (rp189427) - SDS-PAGE
3 μg/lane of Recombinant Human TrkA/NTRK1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56.6-89.3 kDa under reducing conditions and 54.2-84.1 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0622965	分析证书	rp189427
ZJ25F0622964	分析证书	rp189427
ZJ25F0622963	分析证书	rp189427

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