NeutrAvidin Protein (TRITC)

COA

级别和纯度:

1.0 mg/mL

生物活性: Binding Biotin

有货

库存信息

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库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp188521-10μg	10μg	现货
rp188521-100μg	100μg	现货
rp188521-1mg	1mg	现货

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NA (4) NeutrAvidin Protein (4) NeutrAvidin Protein (TRITC) (1) TRITC (16)

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基本描述

产品名称	NeutrAvidin Protein (TRITC)
别名	中性亲和素蛋白 \| NeutrAvidin 蛋白，TRITC \| 亲和素，NeutrAvidin，四甲基异硫氰酸荧光素偶联物 \| 中性亲和素蛋白 (TRITC)
英文别名	Nuetral Avidin \| NeutrAvidin \| NeutrAvidin - TRITC Conjugated
规格或纯度	ExactAb™, 已验证, Ex:557nm, Em:576nm, 1.0 mg/mL
产品介绍	NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding. Features include: • Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin • Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin • No RYD recognition sequence—no known off-target binding domains like streptavidin • Affordable—significantly less expensive than streptavidin NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
生物活性	Binding Biotin
偶联	TRITC
Excitation(nm)	Green Laser(532nm)
Emission(nm)	576nm
Ex/Em(nm)	Ex:557nm;Em:576nm
来源	天然
预测分子量	60kDa

储存与运输

物理形态	液体
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% ProClin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	未稀释的抗体溶液应保存在 2°C 至 8°C 之间，避免长时间暴露在光线下。切勿冷冻。
分子类型	蛋白质

图片

IF: NeutrAvidin Protein (TRITC) (rp188521)

NeutrAvidin Protein (TRITC) (rp188521) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 0.5 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with NeutrAvidin Protein (TRITC) (rp188521) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: NeutrAvidin Protein (TRITC) (rp188521)

NeutrAvidin Protein (TRITC) (rp188521) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.05μg/test for 1 hour at 4°C. Then incubated with NeutrAvidin Protein (TRITC) (rp188521) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
IF/ICC	1:1000
Flow Cytometry	≤0.1 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1012222	分析证书	rp188521
ZJ24F1012223	分析证书	rp188521
ZJ24F1012224	分析证书	rp188521

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