NeutrAvidin Protein (HRP)

COA

级别和纯度:

已验证

ExactAb™

1.0 mg/mL

生物活性: Binding Biotin

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp188518-10μg	10μg	现货
rp188518-100μg	100μg	现货
rp188518-1mg	1mg	期货
rp188518-2mg	2mg	期货

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HRP (36) NA (4) NeutrAvidin Protein (4) NeutrAvidin Protein (HRP) (1)

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基本描述

产品名称	NeutrAvidin Protein (HRP)
别名	中性亲和素蛋白 \| NeutrAvidin 蛋白, HRP \| 亲和素, NeutrAvidin \| 辣根过氧化物酶偶联物
英文别名	Nuetral Avidin \| NeutrAvidin \| NeutrAvidin - Horseradish Peroxidase Conjugated
规格或纯度	ExactAb™, 已验证, 1.0 mg/mL
产品介绍	NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding. Features include: • Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin • Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin • No RYD recognition sequence—no known off-target binding domains like streptavidin • Affordable—significantly less expensive than streptavidin NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
生物活性	Binding Biotin
偶联	HRP
来源	天然
预测分子量	60kDa

储存与运输

物理形态	液体
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% ProClin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20℃ 下可保存一年。避免反复冻融循环。避免光照。
分子类型	蛋白质

图片

Western Blot: NeutrAvidin Protein (HRP) (rp188518)

NeutrAvidin Protein (HRP) (rp188518) - Western Blot
All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: NeutrAvidin Protein (HRP) (rp188518) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 3.0 s

应用

应用名称	稀释比例
WB	1/5000-1/80000
ELISA	1:5000-1:50000

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0911151	分析证书	rp188518
ZJ24F0911152	分析证书	rp188518
ZJ24F0911120	分析证书	rp188518

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