Recombinant Rat PDGF-BB Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)
See COA

表达系统: E. coli
Accession #: Q05028
蛋白标签: No tag
生物活性: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.78 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp186540-10μg	10μg	期货
rp186540-50μg	50μg	期货
rp186540-100μg	100μg	期货
rp186540-1mg	1mg	期货

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点击查看蛋白信息: PDGFB

Accession#: Q05028 (1) Gene ID: 24628 (1) PDGF-BB (2) PDGFB (5)

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基本描述

产品名称	Recombinant Rat PDGF-BB Protein
规格或纯度	无载体,生物活性,ActiBioPure™,≥95%(SDS-PAGE),See COA
生化机理	PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
生物活性	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.78 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	大鼠(Rat)
氨基酸	74-182
序列	SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPVFKKATVTLEDHLACKCETVVTPRPVT
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q05028
来源	重组表达
预测分子量	12.2 kDa
SDS-PAGE	13.5 kDa, under reducing conditions; 29.8 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4.
浓度	See COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Rat PDGF-BB Protein (rp186540)

Recombinant Rat PDGF-BB Protein (rp186540) - Protein Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.78 ng/mL.

SDS-PAGE: Recombinant Rat PDGF-BB Protein (rp186540)

Recombinant Rat PDGF-BB Protein (rp186540) - SDS-PAGE

1 μg/lane of Recombinant Rat PDGF-BB Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.5 kDa under reducing conditions and 29.8 kDa under non-reducing conditions. Rat PDGF-BB are disulfide-linked Antiparallel homodimer.PDGFRα can be activated by homodimeric PDGF-AA, PDGF-BB, and PDGF-CC, and heterodimeric PDGF-AB (PMID: 20534510).

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