Recombinant Canine IL-2 (C147S) Protein

COA

级别和纯度:

≥90%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: Q29416
蛋白标签: No tag
生物活性: Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is 18.45 ng/mL.
内毒素水平: <0.1 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp186528-10μg	10μg	现货
rp186528-50μg	50μg	现货
rp186528-100μg	100μg	现货
rp186528-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL2

Accession#: Q29416 (1) Gene ID: 403989 (1) IL-2 (4) Il2 (7)

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基本描述

产品名称	Recombinant Canine IL-2 (C147S) Protein
别名	重组狗IL-2蛋白 \| 重组狗白细胞介素2蛋白
英文别名	Aldesleukin \| IL2 \| IL-2 \| IL-2lymphokine \| interleukin 2 \| interleukin-2 \| involved in regulation of T-cell clonal expansion \| Proleukin \| T cell growth factor \| T-cell growth factor \| TCGF
规格或纯度	无载体, 生物活性, ActiBioPure™, 高性能, ≥90%(SDS-PAGE), 见COA
生化机理	Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes. The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits. IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9. At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively.
生物活性	Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is 18.45 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	狗(Canine)
氨基酸	21-155 aa (C147S)
序列	APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q29416
来源	重组表达
预测分子量	15.4 kDa
SDS-PAGE	13.3 kDa, under reducing conditions; 11.9 & 26.3 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ for 12 months.Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

Bioactivity: Recombinant Canine IL-2 (C47S) Protein (rp186528)

Recombinant Canine IL-2 (C147S) Protein (rp186528) - Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is 18.45 ng/mL.

SDS-PAGE: Recombinant Canine IL-2 (C147S) Protein (rp186528)

Recombinant Canine IL-2 (C147S) Protein (rp186528) - SDS-PAGE
3 μg/lane of Recombinant Canine IL-2 (C147S) Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.3 kDa under reducing conditions and 11.9 & 26.3 kDa under non-reducing conditions.
Under specific non-physiological conditions, including high concentrations or particular buffer environments, IL-2 monomers have the potential to form non-covalent dimers or higher-order oligomers through non-covalent interactions (Uniprot ID: Q29416).

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0826107	分析证书	rp186528
ZJ25F0826106	分析证书	rp186528
ZJ25F0826105	分析证书	rp186528

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