Recombinant Equine IL-1 beta/IL-1F2 Protein

COA

级别和纯度:

无载体

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q28386
蛋白标签: No tag
生物活性: Testing in progress
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp186525-10μg	10μg	现货
rp186525-50μg	50μg	现货
rp186525-100μg	100μg	现货
rp186525-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: IL1B

Accession#: Q28386 (1) Equine IL-1 beta/IL-1F2 (1) Gene ID: 100034237 (1) Gene ID: 100052414 (1) IL1B (14)

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基本描述

产品名称	Recombinant Equine IL-1 beta/IL-1F2 Protein
别名	重组马 IL-1 beta/IL-1F2蛋白 \| 重组马白细胞介素-1β蛋白
英文别名	catabolin \| IL1 beta \| IL-1 beta \| IL-1 \| IL1B \| IL-1b \| IL1-BETA \| IL-1F2 \| IL1F2IL-1 beta \| interleukin 1, beta \| interleukin-1 beta \| preinterleukin 1 beta \| pro-interleukin-1-beta
规格或纯度	无载体, ≥95%(SDS-PAGE)
生化机理	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form. The 17 kDa mature equine IL-1 beta shares 65% - 75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	马(Horse)
氨基酸	115-268 aa (E179G, M188T, T194I, S245K, R256Q)
序列	DAAMHSVNCRLRDIYHKSLVLSGACELQAVHLNGENTNQQVVFCMSFVQGEEETDKIPVALGLKGKNLYLSCGTKDGKPILQLETVDPNTYPKRKMEKRFVFNKMEIKGNVEFESAMYPNWYISTSQAEKKPVFLGNTRGGQDITDFIMEITSA
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q28386
来源	重组表达
预测分子量	17.3 kDa
SDS-PAGE	14.5 kDa, under reducing conditions; 14.5 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Equine IL-1 beta/IL-1F2 Protein (rp186525)

Recombinant Equine IL-1 beta/IL-1F2 Protein (rp186525) - SDS-PAGE
3 μg/lane of Recombinant Equine IL-1 beta/IL-1F2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.5 kDa.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215827	分析证书	rp186525
ZJ24F1215828	分析证书	rp186525
ZJ24F1215829	分析证书	rp186525
ZJ24F1215830	分析证书	rp186525

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