Recombinant Human ERK2 Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥90%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P28482
蛋白标签: No tag
生物活性: Immobilized Recombinant Human ERK2/MAPK1 Protein (rp184960) at 1.0 μg/mL can bind Recombinant ERK2 Antibody (Ab102233) with the EC50 of 3.3 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp184960-10μg	10μg	现货
rp184960-50μg	50μg	现货
rp184960-100μg	100μg	现货
rp184960-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: MAPK1

Accession#:P28482 (3) ERK2 (3) Gene ID:5594 (3) MAPK1 (3)

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基本描述

产品名称	Recombinant Human ERK2 Protein
别名	重组人ERK2蛋白 \| 重组人有丝分裂原活化蛋白激酶1蛋白
英文别名	EC 2.7.11 \| EC 2.7.11.24 \| ERK \| ERK2 \| ERK-2 \| ERK2MAP kinase isoform p42 \| ERT1 \| Extracellular signal-regulated kinase 2 \| MAP kinase 1 \| MAP kinase 2 \| MAPK 1 \| MAPK1 \| MAPK2 \| mitogen-activated protein kinase 1 \| Mitogen-activated protein kinase 2 \|
规格或纯度	无载体, 生物活性, ActiBioPure™, ≥90%(SDS-PAGE), 见COA
生化机理	ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines. Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta ). Post-translational: Phosphorylated upon KIT and FLT3 signaling (By similarity).Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. Dephosphorylated by DUSP1 and DUSP2 at Thr-185 and Tyr-187 (By similarity) (PubMed:16288922). ISGylated. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation. Deubiquitinated by CYLD.
生物活性	Immobilized Recombinant Human ERK2/MAPK1 Protein (rp184960) at 1.0 μg/mL can bind Recombinant ERK2 Antibody (Ab102233) with the EC50 of 3.3 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-360 aa
序列	AAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	P28482
来源	重组表达
预测分子量	41.2 kDa
SDS-PAGE	38.6 kDa, under reducing conditions; 38.6 kDa, under non-reducing conditions.

储存与运输

物理形态	液体
储存缓冲液	PBS, 5mM DTT, 5% Glycerol, 8% Trehalose, pH 7.4
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

ELISA: Recombinant Human ERK2 Protein (rp184960)

Recombinant Human ERK2 Protein (rp184960) - ELISA
Immobilized Recombinant Human ERK2 Protein (rp184960) at 1.0 μg/mL can bind Recombinant ERK2 Antibody (Ab102233) with the EC50 of 3.3 ng/mL.

SDS-PAGE: Recombinant Human ERK2 Protein (rp189302)

Recombinant Human ERK2 Protein (rp189302) - SDS-PAGE
3 μg/lane of Recombinant Human ERK2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.6 kDa.

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批号(Lot Number)	证书类型	货号
ZJ25F0320016	分析证书	rp184960
ZJ25F0320017	分析证书	rp184960
ZJ25F0320015	分析证书	rp184960
ZJ25F0320014	分析证书	rp184960

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