Recombinant Human DNA-PKcs Protein

COA

级别和纯度:

无载体

≥90%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P78527
蛋白标签: N-His

有货

库存信息

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库存信息

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库存信息

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库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp184914-10μg	10μg	期货
rp184914-50μg	50μg	期货
rp184914-100μg	100μg	期货
rp184914-1mg	1mg	期货

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点击查看蛋白信息: PRKDC

Accession#:P78527 (2) DNA-PKcs (1) Gene ID:5591 (2) PRKDC (2)

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基本描述

产品名称	Recombinant Human DNA-PKcs Protein
别名	重组人DNA-PKcs蛋白 \| 重组人DNA依赖性蛋白激酶催化亚基蛋白
英文别名	DNA dependent protein kinase catalytic subunit \| DNA PK catalytic subunit \| DNA-dependent protein kinase catalytic subunit \| DNA-PK catalytic subunit \| DNA-PKcs \| DNAPK \| DNAPK catalytic subunit \| DNPK 1 \| DNPK1 \| Hyper radiosensitivity of murine scid mut
规格或纯度	无载体, ≥90%(SDS-PAGE), 见COA
生化机理	PRKDC encodes for DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a kinase that forms part of a complex (DNA-dependent protein kinase [DNA-PK]) crucial for DNA double-strand break repair and V(D)J recombination. PRKDC expression was significantly increased in breast cancer tissue samples compared with NATs. High PRKDC expression was associated with higher tumor grade (P=0.001), positive lymph node metastasis (P=0.0357) and chemoresistance (P=0.0006). Furthermore, PRKDC expression was significantly correlated with OS in breast cancer patients with (0.0101) or without (P=0.0216) receiving chemotherapy. Collectively, PRKDC is a prognostic biomarker for chemoresistance in breast cancer patients. Post-translational: Autophosphorylated at two clusters, the T2609 cluster and the S2056 cluster (PubMed:33854234). Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647 (PubMed:12186630, PubMed:12231622, PubMed:14734805, PubMed:33854234). Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C (PubMed:12186630, PubMed:12231622, PubMed:14734805). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair (PubMed:12186630, PubMed:12231622, PubMed:14734805). Autophosphorylation in trans within DNA-PK complexes loaded on DNA ends leads to the dissociation of PRKDC from DNA and the transition into the short-range NHEJ complex (PubMed:33854234). Autophosphorylation of the T2609 cluster is required for hematopoietic development and protein synthesis in erythrocytes precursors (By similarity). S-nitrosylated by GAPDH. Polyubiquitinated by RNF144A, leading to proteasomal degradation.
表达系统	E. coli
种属	人(Human)
氨基酸	3900-4128 aa
序列	MHHHHHHLRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P78527
来源	重组表达
预测分子量	27.0 kDa
SDS-PAGE	27.3 & 53.3 kDa, under reducing conditions

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 500mM NaCl, 1mM DTT, 5% Glycerol, 8M Urea, 50mM Imidazole, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃ 存储6个月；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human DNA-PKcs Protein (rp184914)

Recombinant Human DNA-PKcs Protein (rp184914) - SDS-PAGE
3 μg/lane of Recombinant Human DNA-PKcs Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.3 & 53.3 & 88.1 kDa under reducing conditions and 23.4 & 26.0 & 27.3 & 49.7 & 75.7 kDa under non-reducing conditions.
This expression region contains multiple phosphorylation post-translational modification sites, which may influence its aggregation state. The self phosphorylation of DNA-PKcs induces conformational changes, which leads to the remodeling of the DNA-PK complex. This is essential for efficient end processing and DNA repair (PMID: 33854234).

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