Recombinant Human ATR Protein

COA

级别和纯度:

无载体

≥90%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: Q13535
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp184888-10μg	10μg	期货
rp184888-50μg	50μg	期货
rp184888-100μg	100μg	期货
rp184888-1mg	1mg	期货

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点击查看蛋白信息: ATR

Accession#:Q13535 (1) ATR (2) Gene ID:545 (1)

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基本描述

产品名称	Recombinant Human ATR Protein
别名	重组人ATR蛋白 \| 重组人丝氨酸/苏氨酸蛋白激酶ATR蛋白
英文别名	Ataxia telangiectasia and Rad3 related \| Ataxia telangiectasia and Rad3-related protein \| ATR \| ATR_HUMAN \| FCTCS \| FRAP Related Protein 1 \| FRAP-related protein 1 \| FRP1 \| MEC1 \| MEC1 mitosis entry checkpoint 1 homolog \| Protein kinase ATR \| RAC3 \| Rad3
规格或纯度	无载体, ≥90%(SDS-PAGE), 见COA
生化机理	Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Post-translational: Phosphorylated (PubMed:21144835). Autophosphorylation at Thr-1989 in response to DNA damage promotes interaction with TOPBP1 and activation of ATR (PubMed:21705319, PubMed:21777809).
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2353-2644 aa
序列	MHHHHHHAESRRRELHIRTYAVIPLNDECGIIEWVNNTAGLRPILTKLYKEKGVYMTGKELRQCMLPKSAALSEKLKVFREFLLPRHPPIFHEWFLRTFPDPTSWYSSRSAYCRSTAVMSMVGYILGLGDRHGENILFDSLTGECVHVDFNCLFNKGETFEVPEIVPFRLTHNMVNGMGPMGTEGLFRRACEVTMRLMRDQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKTHVLDIEQRLQGVIKTRNRVTGLPLSIEGHVHYLIQEATDENLLCQMYLGWTPYM
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	Q13535
来源	重组表达
预测分子量	34.5 kDa
SDS-PAGE	30.9 kDa, under reducing conditions; 30.0 & 60.3 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 500mM NaCl, 8M Urea, 166mM Imidazole, pH 8.0.
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human ATR Protein (rp184888)

Recombinant Human ATR Protein (rp184888) - SDS-PAGE
3 μg/lane of Recombinant Human ATR Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.9 kDa under reducing conditions and 30.0 & 60.3 kDa under non-reducing conditions.
ATR Protein can form homodimers through its C-terminal domain, without the involvement of ATRIP (PMID: 35721505).

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