Recombinant Human p70 S6 Kinase Protein

COA

级别和纯度:

无载体

≥85%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P23443
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp184102-10μg	10μg	现货
rp184102-50μg	50μg	现货
rp184102-100μg	100μg	现货
rp184102-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: RPS6KB1

Accession#:P23443 (2) Gene ID:6198 (2) P70 S6 Kinase beta (2) RPS6KB1 (2)

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基本描述

产品名称	Recombinant Human p70 S6 Kinase Protein
别名	重组人p70 S6 Kinase蛋白 \| 重组人核糖体蛋白S6激酶β-1
英文别名	EC 2.7.11 \| EC 2.7.11.1 \| p70 ribosomal S6 kinase alpha \| p70 S6 kinase alpha \| p70 S6 Kinase \| p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1 \| p70 S6 kinase, alpha 2 \| p70 S6KA \| p70 S6K-alpha \| p70(S6K)-alpha \| p70-alpha \| p70-S6K \| P70S6K
规格或纯度	无载体, ≥85%(SDS-PAGE), 见COA
生化机理	p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as at T389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). Post-translational: Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (PubMed:18925875, PubMed:19085255, PubMed:22017876, PubMed:23429703, PubMed:29236692).Activated by phosphorylation at Thr-252 by PDPK1 (PubMed:19864428, PubMed:9445476). Dephosphorylation by PPP1CC at Thr-412 in mitochondrion (PubMed:17936702).
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-525 aa
序列	MHHHHHHRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P23443
来源	重组表达
预测分子量	59.9 kDa
SDS-PAGE	69.0 & 128.0 & 183.4 kDa, under reducing conditions; 65.8 & 70.3 & 120.2 & 183.4 kDa, under non-reducing conditions.

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 500mM NaCl, 1mM DTT, 5% Glycerol, 8M Urea, 48mM Imidazole, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃保存12个月；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human p70 S6 Kinase Protein (rp184102)

Recombinant Human p70 S6 Kinase Protein (rp184102) - SDS-PAGE
2 μg/lane of Recombinant Human p70 S6 Kinase Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 69.0 & 128.0 & 183.4 kDa under reducing conditions and 65.8 & 70.3 & 120.2 & 183.4 kDa under non-reducing conditions.
p70 S6 Kinase activity is highly dependent on phosphorylation. Different phosphorylation states can result in slight differences in molecular weight or changes in migration rate (such as band smearing or slight shifts) (PMID: 23731517).

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ25F0622565	分析证书	rp184102
ZJ25F0622564	分析证书	rp184102
ZJ25F0622567	分析证书	rp184102
ZJ25F0622566	分析证书	rp184102

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