Recombinant Human KMT6/EZH2 Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥80%(SDS-PAGE)

表达系统: E. coli
Accession #: Q15910
蛋白标签: No tag
生物活性: Immobilized Recombinant Human KMT6/EZH2 Protein (rp183733) at 1.0 μg/mL can bind Recombinant KMT6/EZH2 Antibody (Ab112346) with the EC50 of 3.1 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp183733-10μg	10μg	现货
rp183733-50μg	50μg	现货
rp183733-100μg	100μg	现货
rp183733-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: EZH2

Accession#:Q15910 (2) EZH2 (2) Gene ID:2146 (2) KMT6/EZH2 (1)

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基本描述

产品名称	Recombinant Human KMT6/EZH2 Protein
别名	重组人Legumain/Asparaginyl Endopeptidase蛋白 \| 重组人天冬酰胺内肽酶蛋白
英文别名	Enhancer of zeste 2 \| enhancer of zeste 2 polycomb repressive complex 2 subunit \| Enhancer of zeste homolog 2 \| Enhancer of zeste homolog 2 (Drosophila) \| Enhancer of zeste, Drosophila, homolog 2 \| ENX 1 \| Enx 1h \| ENX-1 \| ENX1 \| Enx1h \| EZH 2 \| EZH1 \| EZ
规格或纯度	ActiBioPure™, 生物活性, 无载体, ≥80%(SDS-PAGE)
生化机理	Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. Post-translational: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.
生物活性	Immobilized Recombinant Human KMT6/EZH2 Protein (rp183733) at 1.0 μg/mL can bind Recombinant KMT6/EZH2 Antibody (Ab112346) with the EC50 of 3.1 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-385 aa
序列	GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAG
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q15910
来源	重组表达
预测分子量	44.2 kDa
SDS-PAGE	47.1 kDa, under reducing conditions

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, pH 8.0
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃（12个月）；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human KMT6/EZH2 Protein (rp183733)

Recombinant Human KMT6/EZH2 Protein (rp183733) - SDS-PAGE
2 μg/lane of Recombinant Human KMT6/EZH2 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 47.1 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927825	分析证书	rp183733
ZJ25F0927826	分析证书	rp183733
ZJ25F0927824	分析证书	rp183733

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