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Recombinant Human Superoxide Dismutase 1 Protein

Recombinant Human Superoxide Dismutase 1 Protein

COA

级别和纯度:

无载体

≥95%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P00441
蛋白标签: N-TrxA & His
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp183705-10μg	10μg	现货
rp183705-50μg	50μg	现货
rp183705-100μg	100μg	现货
rp183705-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: SOD1

Accession#: P00441 (5) Gene ID: 6647 (5) SOD1 (6) Superoxide Dismutase 1 (3)

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基本描述

产品名称	Recombinant Human Superoxide Dismutase 1 Protein
别名	重组人SOD1/Superoxide Dismutase 1蛋白 \| 重组人超氧化物歧化酶1蛋白
英文别名	ALS \| ALS1 \| Amyotrophic lateral sclerosis 1 adult \| Cu/Zn SOD \| Cu/Zn superoxide dismutase \| Epididymis secretory protein Li 44 \| HEL S 44 \| Homodimer \| hSod1 \| Indophenoloxidase A \| IPOA \| Mn superoxide dismutase \| SOD \| SOD soluble \| SOD1 \| SOD2 \| SODC
规格或纯度	无载体, ≥95%(SDS-PAGE), 见COA
生化机理	SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. Post-translational: Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-154 aa
序列	MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGHMHHHHHHSSGENLYFQGATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
蛋白标签	N-TrxA & His
无载体	Yes
无动物源	No
Accession #	P00441
来源	重组表达
预测分子量	30.1 kDa
SDS-PAGE	29.3 kDa, under reducing conditions; 29.9 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4.
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	储存-20℃（12个月），复溶后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Superoxide Dismutase 1 Protein (rp183705)

Recombinant Human Superoxide Dismutase 1 Protein (rp183705) - SDS-PAGE
3 μg/lane of Recombinant Human Superoxide Dismutase 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 29.3 kDa under reducing conditions and 29.9 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521566	分析证书	rp183705
ZJ25F0521565	分析证书	rp183705
ZJ25F0521564	分析证书	rp183705

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