Recombinant Human AKT1 Protein

COA

级别和纯度:

无载体

≥70%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P31749
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp183598-10μg	10μg	期货
rp183598-50μg	50μg	期货
rp183598-100μg	100μg	期货
rp183598-1mg	1mg	期货

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点击查看蛋白信息: AKT1

Accession#:P31749 (5) AKT1 (5) Gene ID:207 (5)

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基本描述

产品名称	Recombinant Human AKT1 Protein
别名	重组人AKT1蛋白 \| 重组人RAC-α 丝氨酸/苏氨酸蛋白激酶
英文别名	AKT serine/threonine kinase 1 \| AKT \| Akt1 \| AKT1m \| CWS6 \| EC 2.7.11 \| EC 2.7.11.1 \| PKB alpha \| PKB \| PKBMGC99656 \| PRKBA \| Protein Kinase B Alpha \| Protein kinase B \| Proto-oncogene c-Akt \| rac protein kinase alpha \| RAC \| RAC-alpha serine/threonine-pr
规格或纯度	无载体, ≥70%(SDS-PAGE), 见COA
生化机理	Akt1, also known as PKB alpha, is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility. Akt1 is frequently over-expressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas, and prostate as well as lymphomas and leukemias. Post-translational: O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site. Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity (PubMed:12149249, PubMed:15047712, PubMed:15262962, PubMed:16266983, PubMed:18456494, PubMed:20481595, PubMed:20978158, PubMed:8978681, PubMed:9512493, PubMed:9736715).Phosphorylation of the activation loop at Thr-308 by PDPK1/PDK1 is a prerequisite for full activation (PubMed:9512493). Phosphorylation by mTORC2 in response to growth factors plays a key role in AKT1 activation: mTORC2 phosphorylates different sites depending on the context, such as Thr-450, Ser-473, Ser-477 or Thr-479, thereby facilitating subsequent phosphorylation of the activation loop by PDPK1/PDK1 (PubMed:15718470, PubMed:24670654). Phosphorylation at Ser-473 by mTORC2 promotes ubiquitination and degradation by the proteasome (By similarity). Also phosphorylated at Ser-477 and Thr-479 by CDK2, facilitating subsequent phosphorylation of the activation loop by PDPK1/PDK1 (PubMed:24670654). Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA (PubMed:20333297). This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation (PubMed:20333297). Phosphorylated at Thr-308 and Ser-473 by IKBKE and TBK1 (PubMed:21464307). Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A) (PubMed:14761976). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells (PubMed:17013611). Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (PubMed:16266983). Ser-473 is dephosphorylated by PHLPP (PubMed:28147277). Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase (PubMed:21329884). The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase (PubMed:21329884). Ser-473 is dephosphorylated by CPPED1, leading to termination of signaling (PubMed:23799035). AIM2 acts as an inhibitor of AKT1 by inhibiting phosphorylation Ser-473: AIM2 acts both by inhibiting the activity of PRKDC/DNA-PK kinase and promoting dephosphorylation by PP2A phosphatase (By similarity). Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation (PubMed:19713527). When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated (PubMed:20059950). When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome (PubMed:20059950). Also ubiquitinated by TRIM13 leading to its proteasomal degradation (PubMed:21333377). Phosphorylated, undergoes 'Lys-48'-linked polyubiquitination preferentially at Lys-284 catalyzed by MUL1, leading to its proteasomal degradation (PubMed:22410793). Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome (By similarity). Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition. Cleavage by caspase-3/CASP3 (By similarity). Cleaved at the caspase-3 consensus site Asp-462 during apoptosis, resulting in down-regulation of the AKT signaling pathway and decreased cell survival (PubMed:23152800).
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2-480 aa
序列	MHHHHHHSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P31749
来源	重组表达
预测分子量	56.5 kDa
SDS-PAGE	57.6 kDa, under reducing conditions; 57.6 kDa, under non-reducing conditions.

储存与运输

物理形态	液体
储存缓冲液	20mM Tris, 500mM NaCl, 8M Urea, 10mM DTT, 10mM b-ME, 5% Glycerol, 130mM Imidazole, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃ 存储6个月；收货后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human AKT1 Protein (rp183598)

Recombinant Human AKT1 Protein (rp183598) - SDS-PAGE
3 μg/lane of Recombinant Human AKT1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 57.6 kDa.
Cysteine (Cys) in AKT1 is prone to oxidation during prokaryotic expression and purification, leading to increased degradation. The band observed under the target protein may be a degradation band (Uniprot: P31749).

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