Recombinant Mouse Alpha-synuclein Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: O55042
蛋白标签: C-His
生物活性: Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC50 of 19.8 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185365) with the EC50 of 28.3 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC50 of 475.3 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp183595-10μg	10μg	现货
rp183595-50μg	50μg	现货
rp183595-100μg	100μg	现货
rp183595-1mg	1mg	现货

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点击查看蛋白信息: SNCA

Accession#: O55042 (1) Alpha-synuclein (6) Gene ID: 20617 (1) Snca (7)

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基本描述

产品名称	Recombinant Mouse Alpha-synuclein Protein
别名	重组小鼠Alpha-synuclein蛋白 \| 重组小鼠α突触核蛋白
英文别名	alpha-Synuclein \| I+/--synuclein \| MGC110988 \| NACP \| non A-beta component of AD amyloid \| Non-A beta component of AD amyloid \| Non-A4 component of amyloid precursor \| PARK1 \| PARK4 \| PD1 \| SNCA \| synuclein alpha-140 \| synuclein, alpha (non A4 component o
规格或纯度	ActiBioPure™, 无载体, 生物活性, ≥95%(SDS-PAGE)
生化机理	Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). Besides, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease. Post-translational: Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
生物活性	Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC50 of 19.8 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185365) with the EC50 of 28.3 ng/mL. Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC50 of 475.3 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	小鼠(Mouse)
氨基酸	1-140 aa
序列	MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEAHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	No
Accession #	O55042
来源	重组表达
预测分子量	15.3 kDa
SDS-PAGE	17.1 kDa, under reducing conditions; 17.1 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

ELISA: Recombinant Mouse Alpha-synuclein Protein (rp183595)

Recombinant Mouse Alpha-synuclein Protein (rp183595) - ELISA
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 1.0 μg/mL can bind Alpha-synuclein Mouse mAb (Ab185363) with the EC₅₀ of 19.8 ng/mL.

SDS-PAGE: Recombinant Mouse Alpha-synuclein Protein (rp183595)

Recombinant Mouse Alpha-synuclein Protein (rp183595) - SDS-PAGE
2 μg/lane of Recombinant Mouse Alpha-synuclein Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.1 kDa.

ELISA: Recombinant Mouse Alpha-synuclein Protein (rp183595)

Recombinant Mouse Alpha-synuclein Protein (rp183595) - ELISA
Immobilized Recombinant Mouse Alpha-synuclein Protein (rp183595) at 5.0 μg/mL can bind Cinpanemab (anti-Alpha-synuclein) (Ab176594) with the EC₅₀ of 475.3 ng/mL.

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找到8个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521762	分析证书	rp183595
ZJ25F0521761	分析证书	rp183595
ZJ25F0521760	分析证书	rp183595
ZJ25F0521759	分析证书	rp183595
ZJ24F1215804	分析证书	rp183595
ZJ24F1215803	分析证书	rp183595
ZJ24F1215802	分析证书	rp183595
ZJ24F1215801	分析证书	rp183595

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