Recombinant Streptavidin Protein (AF594)

26篇引用文献

级别和纯度:

1.0 mg/mL

表达系统: E. coli
Accession #: P22629
生物活性: Binding Biotin

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货号 (SKU)	包装规格	是否现货
rp181945-10μg	10μg	现货
rp181945-100μg	100μg	现货
rp181945-1mg	1mg	期货

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Accession#:P22629 (15) AF594 (59) Recombinant Streptavidin Protein (AF594) (1) SA (69) SA-AF594 (1) Streptavidin Protein (46) 链霉亲和素 (71)

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基本描述

产品名称	Recombinant Streptavidin Protein (AF594)
别名	重组链霉亲和素 \| 链霉亲和素
英文别名	SA V1 \| SA V2 \| strepavidin \| Streptavidin V1 \| Streptavidin V2 \| Streptavidin-AF594 \| AF594-Streptavidin \| SA-AF594
规格或纯度	ExactAb™, 已验证, Ex:590nm, Em:618nm, 1.0 mg/mL
生物活性	Binding Biotin
表达系统	E. coli
种属	阿维迪尼链霉菌(Streptomyces avidinii)
无载体	No
无动物源	No
偶联	AF594
Excitation(nm)	Yellow-Green Laser(594nm)
Emission(nm)	618nm
Ex/Em(nm)	Ex:590nm;Em:618nm
Accession #	P22629
来源	重组表达
预测分子量	19 kDa

储存与运输

物理形态	液体
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% ProClin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	蛋白质

图片

IF: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
IF/ICC	1:500
Flow Cytometry	≤1.0 µg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215850	分析证书	rp181945
ZJ24F1215851	分析证书	rp181945

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