Recombinant Human LAG-3 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P18627
蛋白标签: C-His
生物活性: 1. Immobilized Recombinant Human FGL1 Protein (rp146027) at 0.5 μg/mL can bind Recombinant Human LAG-3 Protein (rp176292) with the EC50 of 36.43 ng/mL. 2. Immobilized Recombinant Human LAG-3 Protein (rp176292) at 1.0 μg/mL can bind Relatlimab (anti-LAG-3/CD223) (Ab169321) with the EC50 of 41.53 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp176292-10μg	10μg	现货
rp176292-50μg	50μg	现货
rp176292-100μg	100μg	现货
rp176292-1mg	1mg	期货

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点击查看蛋白信息: LAG3

Accession#:P18627 (8) Gene ID:3902 (8) LAG-3 (2) LAG3 (8)

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基本描述

产品名称	Recombinant Human LAG-3 Protein
别名	重组人LAG-3蛋白 \| 重组人类 LAG-3 蛋白
英文别名	CD223 \| CD223 antigen \| FDC protein \| LAG-3 \| LAG3 \| Lag3 \| LAG3_HUMAN \| Lymphocyte activating 3 \| Lymphocyte activation gene 3 \| Lymphocyte activation gene 3 protein \| Protein FDC \| lymphocyte-activation gene 3 \| Secreted lymphocyte activation gene 3 pro
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function： LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF). LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling. Human LAG-3 cDNA encodes 525 amino acids (aa) that include a 28 aa signal sequence, a 422 aa extracellular domain (ECD) with four Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. Within the ECD, human LAG-3 shares sequence identity with mouse, rat, porcine, and bovine LAG-3. LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli. A soluble 54 kDa form, sLAG-3, can be shed by metalloproteinases ADAM10 and TACE/ADAM17. While monomeric sLAG-3 itself may be inactive, shedding allows for normal T cell activation by removing negative regulation. Binding of a homodimerized sLAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC, and secretion of cytokines such as IFN-gamma and TNF-alpha by type 1 cytotoxic CD8+ T cells and NK cells. sLAG-3/Ig has been used as a potential adjuvant to stimulate a cytotoxic anti-cancer immune response. In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases. In humans, antibody-mediated down regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes. LAG-3 is an immune checkpoint protein that modulates T-cell activation and homeostasis and is a promising target for cancer immunotherapy.
生物活性	1. Immobilized Recombinant Human FGL1 Protein (rp146027) at 0.5 μg/mL can bind Recombinant Human LAG-3 Protein (rp176292) with the EC50 of 36.43 ng/mL. 2. Immobilized Recombinant Human LAG-3 Protein (rp176292) at 1.0 μg/mL can bind Relatlimab (anti-LAG-3/CD223) (Ab169321) with the EC50 of 41.53 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-450 aa
序列	MWEAQFLGLLFLQPLWVAPVKPLQPGAEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHLHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P18627
来源	重组表达
预测分子量	47.0 kDa
SDS-PAGE	57.5 kDa, under reducing condition; 52.3 kDa, under non-reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Binding Activity: Recombinant Human LAG-3 Protein (rp176292)

Recombinant Human LAG-3 Protein (rp176292) - Binding Activity
Immobilized Recombinant Human FGL1 Protein (rp146027) at 0.5 μg/mL can bind Recombinant Human LAG-3 Protein (rp176292) with the EC₅₀ of 36.43 ng/mL.

ELISA: Recombinant Human LAG-3 Protein (rp176292)

Recombinant Human LAG-3 Protein (rp176292) - ELISA
Immobilized Recombinant Human LAG-3 Protein (rp176292) at 1.0 μg/mL can bind Relatlimab (anti-LAG-3/CD223) (Ab169321) with the EC₅₀ of 41.53 ng/mL.

SDS-PAGE: Recombinant Human LAG-3 Protein (rp176292)

Recombinant Human LAG-3 Protein (rp176292) - SDS-PAGE
3 μg/lane of Recombinant Human LAG-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 57.5 kDa under reducing conditions and 52.3 kDa under non-reducing conditions.

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批号(Lot Number)	证书类型	货号
ZJ24F0707678	分析证书	rp176292
ZJ24F0707676	分析证书	rp176292
ZJ24F0707677	分析证书	rp176292

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