Recombinant Human PD-1 Protein

COA

级别和纯度:

无动物源

无载体

生物活性

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q15116
蛋白标签: C-His
生物活性: 1. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Toripalimab (anti-PD-1) (Ab182801) with the EC50 of 13.47 ng/mL. 2. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC50 of 19.95 ng/mL. 3. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Retifanlimab (anti-PD-1) (Ab175644) with the EC50 of 11.39 ng/mL. 4. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Dostarlimab (anti-PD-1) (Ab175513) with the EC50 of 32.46 ng/mL. 5. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Spartalizumab (anti-PD-1) (Ab169299) with the EC50 of 12.83 ng/mL. 6. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Camrelizumab (anti-PD-1) (C412007) with the EC50 of 10.39 ng/mL. 7. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Sintilimab (anti-PD-1) (Ab169307) with the EC50 of 15.02 ng/mL. 8. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Cemiplimab (anti-PD-1) (C412010) with the EC50 of 12.82 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp176241-10μg	10μg	现货
rp176241-50μg	50μg	现货
rp176241-100μg	100μg	现货
rp176241-1mg	1mg	期货

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点击查看蛋白信息: PDCD1

Accession#:Q15116 (39) Gene ID:5133 (39) PD-1 (10) PDCD1 (40)

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基本描述

产品名称	Recombinant Human PD-1 Protein
别名	重组人PD-1蛋白 \| 重组人PD-1蛋白
英文别名	PDCD1_HUMAN \| Programmed cell death 1 \| Programmed cell death 1 protein \| Programmed cell death protein 1 \| Protein PD 1 \| Protein PD-1 \| SLEB2 \| Systemic lupus erythematosus susceptibility 2 \| CD279 \| CD279 antigen \| hPD 1 \| hPD l \| hPD-1 \| hSLE1 \| PD 1
规格或纯度	Animal Free, Carrier Free, Bioactive, ≥95%(SDS-PAGE)
产品介绍	Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Programmed Death-1 receptor (PD-1), also known as CD279, is a type I transmembrane protein belonging to the CD28 family of immune regulatory receptors. Other members of this family include CD28, CTLA-4, ICOS, and BTLA. Mature human PD-1 consists of a 148 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The human PD-1 ECD shares 65% aa sequence identity with the mouse PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion. Thus, the PD-1: PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance. Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy.
生物活性	1. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Toripalimab (anti-PD-1) (Ab182801) with the EC50 of 13.47 ng/mL. 2. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC50 of 19.95 ng/mL. 3. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Retifanlimab (anti-PD-1) (Ab175644) with the EC50 of 11.39 ng/mL. 4. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Dostarlimab (anti-PD-1) (Ab175513) with the EC50 of 32.46 ng/mL. 5. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Spartalizumab (anti-PD-1) (Ab169299) with the EC50 of 12.83 ng/mL. 6. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Camrelizumab (anti-PD-1) (C412007) with the EC50 of 10.39 ng/mL. 7. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Sintilimab (anti-PD-1) (Ab169307) with the EC50 of 15.02 ng/mL. 8. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Cemiplimab (anti-PD-1) (C412010) with the EC50 of 12.82 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-167 aa
序列	MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q15116
来源	重组表达
预测分子量	16.9 kDa
SDS-PAGE	38.6-44.2 kDa, under reducing conditions; 37.0-43.5 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

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