Recombinant Feline CD3 Protein

COA

级别和纯度:

≥90%(SDS-PAGE)
见COA

表达系统: HEK293
Accession #: Q5R1Q1
蛋白标签: C-His
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp175929-10μg	10μg	现货
rp175929-50μg	50μg	现货
rp175929-100μg	100μg	现货
rp175929-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD3E

Accession#: Q5R1Q1 (1) CD3 (46) CD3E (62) Gene ID: 4493936 (1)

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基本描述

产品名称	Recombinant Feline CD3 Protein
别名	重组猫CD3 epsilon蛋白 \| 重组猫T细胞表面糖蛋白CD3ε链蛋白
英文别名	CD3 epsilon \| CD3e \| CD3e antigen \| CD3E antigen epsilon polypeptide \| CD3e antigen epsilon polypeptide (TiT3 complex) \| CD3E antigen, epsilon subunit \| CD3e molecule epsilon \| CD3e molecule, epsilon (CD3 TCR complex) \| CD3e molecule, epsilon (CD3-TCR com
规格或纯度	无动物源, 无载体, 生物活性, ActiBioPure™, ≥90%(SDS-PAGE), 见COA
生化机理	CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain. With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3δ, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement. CD3 proteins form heterodimers of CD3δ/CD3 epsilon and CD3 gamma /CD3 epsilon, which bind to TCR and form trimeric TCR alpha /CD3 epsilon /CD3 gamma and TCR beta /CD3 gamma /CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex. Similar complex is also formed with TCR gamma and TCRδ. CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency). Post-translational: Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	猫(Feline)
氨基酸	22-125 aa
序列	QEDNEDPLEPSPQTSASARYKVSISGTTVVLTCPEDLGSESIKWERNGDLLPNEYGEQLFLDDFSEMENSGYYACYTSNSLEKNYLYLKARVCQNCVEVDTMTAHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q5R1Q1
来源	重组表达
预测分子量	12.5 kDa
SDS-PAGE	14.7 kDa, under reducing conditions; 15.8 & 26.4 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	储存-20℃（12个月），复溶后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Cat CD3 Protein (rp175929)

Recombinant Cat CD3 Protein (rp175929) - SDS-PAGE
3 μg/lane of Recombinant Cat CD3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.7 kDa under reducing conditions and 15.8 & 26.4 kDa under non-reducing conditions.
The complex assembled in the ER contains only a single TCRαβ heterodimer and one copy of each of the CD3δε, CD3γε and ζ–ζ signaling dimers (PMID: 15072848).

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521950	分析证书	rp175929
ZJ25F0521951	分析证书	rp175929
ZJ25F0521952	分析证书	rp175929

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