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Recombinant Human HRV 3C Protein

Recombinant Human HRV 3C Protein

级别和纯度:

≥95%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P03303
蛋白标签: N-His
生物活性: 2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
rp170396-1KU	1KU	现货

查看相关系列

Accession#:P03303 (1) Gene ID:1461213 (1) HRV 3C (1) HRVBgp1 (1)

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基本描述

产品名称	Recombinant Human HRV 3C Protein
别名	重组人HRV 3C蛋白 \| 重组人HRV 3C蛋白
英文别名	HRV-3C Protease \| 3C Protease
规格或纯度	无载体, 生物活性, ActiBioPure™, 无叠氮钠, ≥95%(SDS-PAGE), 见COA
产品介绍	HRV 3C蛋白酶是人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶，在大肠杆菌中表达并纯化。HRV 3C蛋白酶在Gln和Gly残基之间以识别序列Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro切割蛋白质底物。该蛋白酶的高特异性和高亲和力标签(6xHis)使其成为去除重组蛋白纯化和检测标签的理想选择，并能灵活去除该蛋白酶。来源： HRV 3C蛋白酶是在大肠杆菌中表达并纯化的一种人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶。 HRV 3C酶切His-GST-IL33蛋白，按照质量比（HRV 3C：目的蛋白）1：25和1：50酶切，4℃过夜酶切结果如下：完全酶切干净。 Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C)expressed in and purified from Escherichia coli. HRV 3C Protease cleaves protein substrates with the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro between the Gln and Gly residues. The high specificity and affinity tags( 6xHis) of the protease make it an ideal choice for the removal of purification and detection tags on recombinant proteins and allows for flexibility in protease removal. Source： HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C) expressed in and purified from Escherichia coli. HRV 3C enzyme digestion of His-GST-IL33 protein, according to the mass ratio (HRV 3C: target protein) 1:25 and 1:50 enzyme digestion, overnight at 4℃ enzyme digestion results are as follows: completely clean enzyme digestion
生物活性	2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	1538-1719 aa
序列	MGSSHHHHHHSSGLVPRGSHMGPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKLVDPENINLELTVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDYATKTGQCGGVLCATGKIFGIHVGGNGRQGFSAQLKKQYFVEKQ.
纯度	>95% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P03303
来源	重组表达
预测分子量	22.3 kDa
SDS-PAGE	24.0 kDa, reducing conditions; 24.0 kDa, non-reducing conditions

储存与运输

储存缓冲液	10mM PBS, pH7.4
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	-20℃保存12个月; 收货后建议分装 , 避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant HRV 3C Protease Protein (rp170396)

Recombinant HRV 3C Protease Protein (rp170396) - SDS-PAGE
3 μg/lane of Recombinant HRV 3C Protease Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 24.0 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ25F0421091	分析证书	rp170396
ZJ23F1202038	分析证书	rp170396

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