Recombinant Human PD-L1 Protein

COA

级别和纯度:

无动物源

无载体

生物活性

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q9NZQ7
蛋白标签: C-His
生物活性: Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC50 of 169.70 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC50 of 77.96 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC50 of 339.40 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC50 of 35.40 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp169666-10μg	10μg	现货
rp169666-50μg	50μg	现货
rp169666-100μg	100μg	现货
rp169666-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: CD274

Accession#:Q9NZQ7 (26) CD274 (23) Gene ID:29126 (26) PD-L1 (22)

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基本描述

产品名称	Recombinant Human PD-L1 Protein
别名	重组人PD-L1蛋白 \| 重组人PD-L1蛋白
英文别名	B7 H \| B7 H1 \| B7 homolog 1 \| B7-H1 \| B7H \| B7H1 \| CD 274 \| CD-274 \| CD274 \| CD274 antigen \| CD274 molecule \| MGC142294 \| MGC142296 \| OTTHUMP00000021029 \| PD L1 \| PD-L1 \| PD1L1_HUMAN \| PDCD1 ligand 1 \| PDCD1L1 \| PDCD1LG1 \| PDL 1 \| PDL1 \| Programmed cell d
规格或纯度	Animal Free, Carrier Free, Bioactive, ≥95%(SDS-PAGE)
产品介绍	Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
生物活性	Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC50 of 169.70 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC50 of 77.96 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC50 of 339.40 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC50 of 35.40 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-239 aa
序列	MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q9NZQ7
来源	重组表达
预测分子量	26.1 kDa
SDS-PAGE	30.7-37.3 kDa, under reducing conditions; 28.6-36.2 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human PD-L1 Protein (rp169666)

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC₅₀ of 169.70 ng/mL.

ELISA: Recombinant Human PD-L1 Protein (rp169666)

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC₅₀ of 77.96 ng/mL.

ELISA: Recombinant Human PD-L1 Protein (rp169666)

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC₅₀ of 339.40 ng/mL.

ELISA: Recombinant Human PD-L1 Protein (rp169666)

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC₅₀ of 35.40 ng/mL.

SDS-PAGE: Recombinant Human PD-L1 Protein (rp169666)

Recombinant Human PD-L1 Protein (rp169666) - SDS-PAGE
3 μg/lane of Recombinant Human PD-L1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.7-37.3 kDa under reducing conditions and 28.6-36.2 kDa under non-reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0606445	分析证书	rp169666
ZJ24F0606442	分析证书	rp169666
ZJ24F0606443	分析证书	rp169666
ZJ24F0606444	分析证书	rp169666

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