Recombinant Human CASP-1 Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: P29466
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp169566-10μg	10μg	现货
rp169566-100μg	100μg	现货
rp169566-1mg	1mg	现货

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点击查看蛋白信息: CASP1

Accession#:P29466 (4) CASP-1 (1) CASP1 (4) Gene ID:834 (4)

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基本描述

产品名称	Recombinant Human CASP-1 Protein
别名	重组人CASP-1蛋白 \| 重组人CASP-1蛋白
英文别名	CASP-1 \| CASP1 \| CASP1_HUMAN \| Caspase 1 \| Caspase-1 subunit p10 \| ICE \| IL-1 beta-converting enzyme \| IL-1BC \| IL1 beta converting enzyme \| IL1B convertase \| Interleukin 1 beta convertase \| Interleukin 1B converting enzyme \| Interleukin-1 beta convertase
规格或纯度	Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Caspase-1/CASP1 subunit p10 Protein, a thiol protease, intricately participates in various inflammatory processes by catalyzing the proteolytic cleavage of precursor proteins associated with inflammation. This includes interleukin-1 beta (IL1B) and interleukin 18 (IL18), pivotal inflammatory cytokines, as well as the pyroptosis inducer Gasdermin-D (GSDMD), converting them into active mature peptides. Functioning as a key initiator of cell immunity, Caspase-1 activates a pro-inflammatory response upon inflammasome complex formation, leading to the release of mature cytokines IL1B and IL18, which play critical roles in diverse inflammatory pathways. Caspase-1's activity extends to initiating pyroptosis, a programmed lytic cell death pathway, by cleaving GSDMD. Notably, unlike its cleavage of interleukins, the recognition and cleavage of GSDMD depend on an exosite interface on CASP1, emphasizing its versatile and complex regulatory roles. Moreover, Caspase-1 activates CASP7 in response to bacterial infection, promoting plasma membrane repair, and controls antiviral immunity by cleaving CGAS upon inflammasome activation during DNA virus infection. In apoptotic cells, Caspase-1 cleaves SPHK2, which remains enzymatically active extracellularly. Despite its involvement in diverse cellular processes, Caspase-1 is apoptosis inactive.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	120-297 aa
序列	MHHHHHHNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKD
纯度	>90% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P29466
来源	重组表达
预测分子量	20.7 kDa
SDS-PAGE	20.5 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 500mM NaCl, 8M Urea, 20mM Imidazole, pH 8.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human CASP-1 Protein (rp169566)

Recombinant Human CASP-1 Protein (rp169566) - SDS-PAGE
3 μg/lane of Recombinant Human CASP-1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 20.5 kDa.

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批号(Lot Number)	证书类型	货号
ZJ24F0404979	分析证书	rp169566
ZJ24F0404980	分析证书	rp169566
ZJ24F0404981	分析证书	rp169566

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