Recombinant Human ASGR1/ASGPR1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P07306
蛋白标签: N-His
生物活性: Measured by its binding ability in a functional ELISA. SARS-CoV-2 Spike BRD (rp155929) at 0.5 μg/mL can bind Human ASGR1/ASGPR1(biotin) (rp169552) with the EC50 of 491.2 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp169552-10μg	10μg	现货
rp169552-50μg	50μg	现货
rp169552-100μg	100μg	期货
rp169552-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: ASGR1

Accession#:P07306 (1) ASGPR1 (1) ASGR1 (1) Gene ID:432 (1)

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基本描述

产品名称	Recombinant Human ASGR1/ASGPR1 Protein
别名	重组人ASGR1/ASGPR1蛋白 \| 重组人 ASGR1/ASGPR1 蛋白
英文别名	ASGP-R 1 \| ASGPR \| ASGPR 1 \| ASGPR1 \| Asgr1 \| ASGR1 \| ASGR1_HUMAN \| Asialoglycoprotein receptor 1 \| C type lectin domain family 4 member H1 \| C-type lectin domain family 4 member H1 \| CLEC4H1 \| Hepatic lectin H1 \| HL-1 \| MHL1 \| RHL1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
生物活性	Measured by its binding ability in a functional ELISA. SARS-CoV-2 Spike BRD (rp155929) at 0.5 μg/mL can bind Human ASGR1/ASGPR1(biotin) (rp169552) with the EC50 of 491.2 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	62-291 aa
序列	HHHHHHQNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
纯度	>95% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	Yes
Accession #	P07306
来源	重组表达
预测分子量	27.2 kDa
SDS-PAGE	38.0 kDa, under reducing conditions; 35.8 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human ASGR1/ASGPR1 Protein (rp169552)

Recombinant Human ASGR1/ASGPR1 Protein (rp169552) - ELISA

Measured by its binding ability in a functional ELISA. SARS-CoV-2 Spike BRD (rp155929) at 0.5 μg/mL can bind Human ASGR1/ASGPR1(biotin) (rp169552) with the EC₅₀ of 491.2 ng/mL.

SDS-PAGE: Recombinant Human ASGR1/ASGPR1 Protein (rp169552)

Recombinant Human ASGR1/ASGPR1 Protein (rp169552) - SDS-PAGE
2 μg/lane of Recombinant Human ASGR1/ASGPR1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.0 kDa.

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404523	分析证书	rp169552
ZJ24F0404524	分析证书	rp169552

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